Matches in SemOpenAlex for { <https://semopenalex.org/work/W2076755093> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2076755093 endingPage "17" @default.
- W2076755093 startingPage "12" @default.
- W2076755093 abstract "Thermostability can be increased by introducing prolines at suitable sites in target proteins. In this study, we compared five thermostable α-glucosidases and the moderate thermostable α-glucosidase (TtGluA) from Thermoanaerobacter tengcongensis MB4. Based on the amino acid sequence alignment, four sites (Leu152, Asn208, Lys285, and Thr430) of TtGluA were chosen for proline substitution to improve its thermostability. Thermostability of mutants L152P, K285P, and T430P increased evidently, but no thermostability improvement was observed for N208P. Compared to the wild-type enzyme, T5015 of T430P had a rise of 2 °C without distinct loss of activity. However, T5015 values of L152P and K285P increased 2 °C and 10.5 °C, respectively, while retaining activity of only 26.6% and 24.9% of wild-type enzyme. The Km of L152P, K285P, T430P and wild-type enzyme was 1.61, 0.32, 1.64, and 1.08 mM, respectively. These indicate that the selected sites are not only important for the thermostability but also related to the substrate binding and catalytic activity of TtGluA. The CD spectra analysis of the improved mutants and wild-type enzyme showed no distinct changes in their secondary structures. Combining analysis of secondary structure prediction and 3D structure modeling, the proline substitution at the three sites stabilized TtGluA possibly by reducing the flexibility of loop and random coil or (and) increasing the hydrophobic effect at these strategic regions with no evident structure change." @default.
- W2076755093 created "2016-06-24" @default.
- W2076755093 creator A5038264400 @default.
- W2076755093 creator A5082772250 @default.
- W2076755093 creator A5085591303 @default.
- W2076755093 date "2010-07-01" @default.
- W2076755093 modified "2023-10-18" @default.
- W2076755093 title "Enhancing the thermostability of α-glucosidase from Thermoanaerobacter tengcongensis MB4 by single proline substitution" @default.
- W2076755093 cites W1541189253 @default.
- W2076755093 cites W1549709155 @default.
- W2076755093 cites W1565223565 @default.
- W2076755093 cites W1975865706 @default.
- W2076755093 cites W1995180700 @default.
- W2076755093 cites W2001127398 @default.
- W2076755093 cites W2001210310 @default.
- W2076755093 cites W2031778321 @default.
- W2076755093 cites W2044566017 @default.
- W2076755093 cites W2047572594 @default.
- W2076755093 cites W2073962404 @default.
- W2076755093 cites W2083354414 @default.
- W2076755093 cites W2088895682 @default.
- W2076755093 cites W2092390674 @default.
- W2076755093 cites W2106269045 @default.
- W2076755093 cites W2108320685 @default.
- W2076755093 cites W2112207535 @default.
- W2076755093 cites W2127849149 @default.
- W2076755093 cites W2130027714 @default.
- W2076755093 cites W2143078548 @default.
- W2076755093 cites W2143924450 @default.
- W2076755093 cites W2151433688 @default.
- W2076755093 cites W2166801124 @default.
- W2076755093 cites W4234796795 @default.
- W2076755093 cites W4256308970 @default.
- W2076755093 cites W4318443743 @default.
- W2076755093 doi "https://doi.org/10.1016/j.jbiosc.2009.12.002" @default.
- W2076755093 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20541109" @default.
- W2076755093 hasPublicationYear "2010" @default.
- W2076755093 type Work @default.
- W2076755093 sameAs 2076755093 @default.
- W2076755093 citedByCount "54" @default.
- W2076755093 countsByYear W20767550932012 @default.
- W2076755093 countsByYear W20767550932013 @default.
- W2076755093 countsByYear W20767550932014 @default.
- W2076755093 countsByYear W20767550932015 @default.
- W2076755093 countsByYear W20767550932017 @default.
- W2076755093 countsByYear W20767550932018 @default.
- W2076755093 countsByYear W20767550932019 @default.
- W2076755093 countsByYear W20767550932020 @default.
- W2076755093 countsByYear W20767550932021 @default.
- W2076755093 countsByYear W20767550932022 @default.
- W2076755093 countsByYear W20767550932023 @default.
- W2076755093 crossrefType "journal-article" @default.
- W2076755093 hasAuthorship W2076755093A5038264400 @default.
- W2076755093 hasAuthorship W2076755093A5082772250 @default.
- W2076755093 hasAuthorship W2076755093A5085591303 @default.
- W2076755093 hasConcept C104317684 @default.
- W2076755093 hasConcept C143065580 @default.
- W2076755093 hasConcept C147816474 @default.
- W2076755093 hasConcept C181199279 @default.
- W2076755093 hasConcept C185592680 @default.
- W2076755093 hasConcept C2779815552 @default.
- W2076755093 hasConcept C2781216036 @default.
- W2076755093 hasConcept C27911776 @default.
- W2076755093 hasConcept C41183919 @default.
- W2076755093 hasConcept C515207424 @default.
- W2076755093 hasConcept C55493867 @default.
- W2076755093 hasConceptScore W2076755093C104317684 @default.
- W2076755093 hasConceptScore W2076755093C143065580 @default.
- W2076755093 hasConceptScore W2076755093C147816474 @default.
- W2076755093 hasConceptScore W2076755093C181199279 @default.
- W2076755093 hasConceptScore W2076755093C185592680 @default.
- W2076755093 hasConceptScore W2076755093C2779815552 @default.
- W2076755093 hasConceptScore W2076755093C2781216036 @default.
- W2076755093 hasConceptScore W2076755093C27911776 @default.
- W2076755093 hasConceptScore W2076755093C41183919 @default.
- W2076755093 hasConceptScore W2076755093C515207424 @default.
- W2076755093 hasConceptScore W2076755093C55493867 @default.
- W2076755093 hasIssue "1" @default.
- W2076755093 hasLocation W20767550931 @default.
- W2076755093 hasLocation W20767550932 @default.
- W2076755093 hasOpenAccess W2076755093 @default.
- W2076755093 hasPrimaryLocation W20767550931 @default.
- W2076755093 hasRelatedWork W109238282 @default.
- W2076755093 hasRelatedWork W111524047 @default.
- W2076755093 hasRelatedWork W152545776 @default.
- W2076755093 hasRelatedWork W1970190337 @default.
- W2076755093 hasRelatedWork W2005407799 @default.
- W2076755093 hasRelatedWork W2058410375 @default.
- W2076755093 hasRelatedWork W2068147017 @default.
- W2076755093 hasRelatedWork W2073544937 @default.
- W2076755093 hasRelatedWork W2382137666 @default.
- W2076755093 hasRelatedWork W2955670938 @default.
- W2076755093 hasVolume "110" @default.
- W2076755093 isParatext "false" @default.
- W2076755093 isRetracted "false" @default.
- W2076755093 magId "2076755093" @default.
- W2076755093 workType "article" @default.