Matches in SemOpenAlex for { <https://semopenalex.org/work/W2076757110> ?p ?o ?g. }
- W2076757110 endingPage "252" @default.
- W2076757110 startingPage "240" @default.
- W2076757110 abstract "Clavulanic acid is a potent beta-lactamase inhibitor used to combat resistance to penicillin and cephalosporin antibiotics. There is a demand for high-yielding fermentation strains for industrial production of this valuable product. Clavulanic acid biosynthesis is initiated by the condensation of L-arginine and D-glyceraldehyde-3-phosphate (G3P). To overcome the limited G3P pool and improve clavulanic acid production, we genetically engineered the glycolytic pathway in Streptomyces clavuligerus. Two genes (gap1 and gap2) whose protein products are distinct glyceraldehyde-3-phosphate dehydrogenases (GAPDHs) were inactivated in S. clavuligerus by targeted gene disruption. A doubled production of clavulanic acid was consistently obtained when gap1 was disrupted, and reversed by complementation. Addition of arginine to the cultured mutant further improved clavulanic acid production giving a greater than 2-fold increase over wild type, suggesting that arginine became limiting for biosynthesis. This is the first reported application of genetic engineering to channel precursor flux to improve clavulanic acid production." @default.
- W2076757110 created "2016-06-24" @default.
- W2076757110 creator A5067252545 @default.
- W2076757110 creator A5068952473 @default.
- W2076757110 date "2006-05-01" @default.
- W2076757110 modified "2023-10-14" @default.
- W2076757110 title "Rational strain improvement for enhanced clavulanic acid production by genetic engineering of the glycolytic pathway in Streptomyces clavuligerus" @default.
- W2076757110 cites W116904374 @default.
- W2076757110 cites W139441668 @default.
- W2076757110 cites W1514157228 @default.
- W2076757110 cites W1521398910 @default.
- W2076757110 cites W1539580523 @default.
- W2076757110 cites W1902944504 @default.
- W2076757110 cites W1941339364 @default.
- W2076757110 cites W1975117802 @default.
- W2076757110 cites W1977689824 @default.
- W2076757110 cites W1985420890 @default.
- W2076757110 cites W1995995599 @default.
- W2076757110 cites W1997271047 @default.
- W2076757110 cites W1999523287 @default.
- W2076757110 cites W2000168862 @default.
- W2076757110 cites W2003975780 @default.
- W2076757110 cites W2005090163 @default.
- W2076757110 cites W2010885563 @default.
- W2076757110 cites W2012960603 @default.
- W2076757110 cites W2028054320 @default.
- W2076757110 cites W2029651088 @default.
- W2076757110 cites W2035880040 @default.
- W2076757110 cites W2047029681 @default.
- W2076757110 cites W2055934147 @default.
- W2076757110 cites W2058995008 @default.
- W2076757110 cites W2077537756 @default.
- W2076757110 cites W2088021352 @default.
- W2076757110 cites W2101852959 @default.
- W2076757110 cites W2106233702 @default.
- W2076757110 cites W2116304868 @default.
- W2076757110 cites W2120065358 @default.
- W2076757110 cites W2123793235 @default.
- W2076757110 cites W2123815603 @default.
- W2076757110 cites W2124294739 @default.
- W2076757110 cites W2125811524 @default.
- W2076757110 cites W2129349184 @default.
- W2076757110 cites W2130824852 @default.
- W2076757110 cites W2135462873 @default.
- W2076757110 cites W2142500173 @default.
- W2076757110 cites W2146179813 @default.
- W2076757110 cites W2148393598 @default.
- W2076757110 cites W2148464225 @default.
- W2076757110 cites W2151420444 @default.
- W2076757110 cites W2158942980 @default.
- W2076757110 cites W2160637002 @default.
- W2076757110 cites W2253845724 @default.
- W2076757110 cites W265480990 @default.
- W2076757110 cites W641674982 @default.
- W2076757110 doi "https://doi.org/10.1016/j.ymben.2006.01.003" @default.
- W2076757110 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16530442" @default.
- W2076757110 hasPublicationYear "2006" @default.
- W2076757110 type Work @default.
- W2076757110 sameAs 2076757110 @default.
- W2076757110 citedByCount "87" @default.
- W2076757110 countsByYear W20767571102012 @default.
- W2076757110 countsByYear W20767571102013 @default.
- W2076757110 countsByYear W20767571102014 @default.
- W2076757110 countsByYear W20767571102015 @default.
- W2076757110 countsByYear W20767571102016 @default.
- W2076757110 countsByYear W20767571102017 @default.
- W2076757110 countsByYear W20767571102018 @default.
- W2076757110 countsByYear W20767571102019 @default.
- W2076757110 countsByYear W20767571102020 @default.
- W2076757110 countsByYear W20767571102021 @default.
- W2076757110 countsByYear W20767571102022 @default.
- W2076757110 countsByYear W20767571102023 @default.
- W2076757110 crossrefType "journal-article" @default.
- W2076757110 hasAuthorship W2076757110A5067252545 @default.
- W2076757110 hasAuthorship W2076757110A5068952473 @default.
- W2076757110 hasConcept C100544194 @default.
- W2076757110 hasConcept C104317684 @default.
- W2076757110 hasConcept C143065580 @default.
- W2076757110 hasConcept C185592680 @default.
- W2076757110 hasConcept C188082640 @default.
- W2076757110 hasConcept C2777211131 @default.
- W2076757110 hasConcept C2779454058 @default.
- W2076757110 hasConcept C2779708577 @default.
- W2076757110 hasConcept C501593827 @default.
- W2076757110 hasConcept C553450214 @default.
- W2076757110 hasConcept C55493867 @default.
- W2076757110 hasConcept C6350086 @default.
- W2076757110 hasConcept C86803240 @default.
- W2076757110 hasConcept C89423630 @default.
- W2076757110 hasConceptScore W2076757110C100544194 @default.
- W2076757110 hasConceptScore W2076757110C104317684 @default.
- W2076757110 hasConceptScore W2076757110C143065580 @default.
- W2076757110 hasConceptScore W2076757110C185592680 @default.
- W2076757110 hasConceptScore W2076757110C188082640 @default.
- W2076757110 hasConceptScore W2076757110C2777211131 @default.
- W2076757110 hasConceptScore W2076757110C2779454058 @default.
- W2076757110 hasConceptScore W2076757110C2779708577 @default.
- W2076757110 hasConceptScore W2076757110C501593827 @default.