FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2076789453> ?p ?o ?g. }

• W2076789453 endingPage "3645" @default.
• W2076789453 startingPage "3638" @default.
• W2076789453 abstract "A novel signal amplification technology based on a new class of biofunctional fluorescent nanocrystals holds promise to improve the sensitivity and the limits of detection of immunoassays. A two-step approach without layer-by-layer techniques is described to encapsulate the fluorogenic precursor fluorescein diacetate (FDA) nanocrystals (107-nm average size) followed by conjugation of the antibody. Distearoylphosphatidylethanolamine (DSPE) modified with amino(poly(ethylene glycol)) (PEG(2000)Amine) is coated on the surface of the FDA nanocrystals to provide a interface for the antibody coupling. Anti-mouse antibodies are attached to the nanocrystalline FDA biolabels by adsorption. A high molar ratio of fluorescent molecules to biomolecules (2.8 x 10(4)) is achieved in this nanocrystal biolabel system. The analytical performance of the nanocrystal-based label system is evaluated in a model sandwich immunoassay for the detection of mouse IgG. After separation of the nonbound antibody nanocrystal labels, fluorophores are released by hydrolysis and dissolution of the nanocrystalline FDA. Due to the release of the fluorophores (fluoresceins) into a large volume of organic solvent/sodium hydroxide mixture, self-quenching is suppressed. The FDA[DSPE-PEG(2000)Amine]-modified biolabels have a highly stable colloidal suspension with minimized nonspecific interactions. The limit of detection was lowered by a factor of 5-28, and the sensitivity was 400-2700-fold higher compared with a state-of-the-art immunoassay using directly fluorescent-labeled antibodies. Our approach provides high sensitivity and low limits of detection without the need for long incubation times, making it an interesting alternative in biolabel technology." @default.
• W2076789453 created "2016-06-24" @default.
• W2076789453 creator A5019022141 @default.
• W2076789453 creator A5019771525 @default.
• W2076789453 creator A5022289847 @default.
• W2076789453 creator A5041361458 @default.
• W2076789453 creator A5047258004 @default.
• W2076789453 creator A5050951221 @default.
• W2076789453 creator A5071437234 @default.
• W2076789453 creator A5083101941 @default.
• W2076789453 date "2004-05-21" @default.
• W2076789453 modified "2023-10-16" @default.
• W2076789453 title "Nanocrystal Biolabels with Releasable Fluorophores for Immunoassays" @default.
• W2076789453 cites W1968210203 @default.
• W2076789453 cites W1972404567 @default.
• W2076789453 cites W1989340893 @default.
• W2076789453 cites W2028835321 @default.
• W2076789453 cites W2048616824 @default.
• W2076789453 cites W2054064585 @default.
• W2076789453 cites W2054272812 @default.
• W2076789453 cites W2070103790 @default.
• W2076789453 cites W2123504097 @default.
• W2076789453 doi "https://doi.org/10.1021/ac0353740" @default.
• W2076789453 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15228335" @default.
• W2076789453 hasPublicationYear "2004" @default.
• W2076789453 type Work @default.
• W2076789453 sameAs 2076789453 @default.
• W2076789453 citedByCount "73" @default.
• W2076789453 countsByYear W20767894532012 @default.
• W2076789453 countsByYear W20767894532013 @default.
• W2076789453 countsByYear W20767894532014 @default.
• W2076789453 countsByYear W20767894532015 @default.
• W2076789453 countsByYear W20767894532016 @default.
• W2076789453 countsByYear W20767894532017 @default.
• W2076789453 countsByYear W20767894532018 @default.
• W2076789453 countsByYear W20767894532019 @default.
• W2076789453 countsByYear W20767894532020 @default.
• W2076789453 countsByYear W20767894532021 @default.
• W2076789453 countsByYear W20767894532023 @default.
• W2076789453 crossrefType "journal-article" @default.
• W2076789453 hasAuthorship W2076789453A5019022141 @default.
• W2076789453 hasAuthorship W2076789453A5019771525 @default.
• W2076789453 hasAuthorship W2076789453A5022289847 @default.
• W2076789453 hasAuthorship W2076789453A5041361458 @default.
• W2076789453 hasAuthorship W2076789453A5047258004 @default.
• W2076789453 hasAuthorship W2076789453A5050951221 @default.
• W2076789453 hasAuthorship W2076789453A5071437234 @default.
• W2076789453 hasAuthorship W2076789453A5083101941 @default.
• W2076789453 hasConcept C10138342 @default.
• W2076789453 hasConcept C119128265 @default.
• W2076789453 hasConcept C121332964 @default.
• W2076789453 hasConcept C127413603 @default.
• W2076789453 hasConcept C155672457 @default.
• W2076789453 hasConcept C159654299 @default.
• W2076789453 hasConcept C162324750 @default.
• W2076789453 hasConcept C171250308 @default.
• W2076789453 hasConcept C175854130 @default.
• W2076789453 hasConcept C178790620 @default.
• W2076789453 hasConcept C185592680 @default.
• W2076789453 hasConcept C192562407 @default.
• W2076789453 hasConcept C203014093 @default.
• W2076789453 hasConcept C2777516009 @default.
• W2076789453 hasConcept C2780420688 @default.
• W2076789453 hasConcept C42360764 @default.
• W2076789453 hasConcept C43617362 @default.
• W2076789453 hasConcept C49853544 @default.
• W2076789453 hasConcept C54400483 @default.
• W2076789453 hasConcept C55493867 @default.
• W2076789453 hasConcept C62520636 @default.
• W2076789453 hasConcept C86803240 @default.
• W2076789453 hasConcept C91881484 @default.
• W2076789453 hasConceptScore W2076789453C10138342 @default.
• W2076789453 hasConceptScore W2076789453C119128265 @default.
• W2076789453 hasConceptScore W2076789453C121332964 @default.
• W2076789453 hasConceptScore W2076789453C127413603 @default.
• W2076789453 hasConceptScore W2076789453C155672457 @default.
• W2076789453 hasConceptScore W2076789453C159654299 @default.
• W2076789453 hasConceptScore W2076789453C162324750 @default.
• W2076789453 hasConceptScore W2076789453C171250308 @default.
• W2076789453 hasConceptScore W2076789453C175854130 @default.
• W2076789453 hasConceptScore W2076789453C178790620 @default.
• W2076789453 hasConceptScore W2076789453C185592680 @default.
• W2076789453 hasConceptScore W2076789453C192562407 @default.
• W2076789453 hasConceptScore W2076789453C203014093 @default.
• W2076789453 hasConceptScore W2076789453C2777516009 @default.
• W2076789453 hasConceptScore W2076789453C2780420688 @default.
• W2076789453 hasConceptScore W2076789453C42360764 @default.
• W2076789453 hasConceptScore W2076789453C43617362 @default.
• W2076789453 hasConceptScore W2076789453C49853544 @default.
• W2076789453 hasConceptScore W2076789453C54400483 @default.
• W2076789453 hasConceptScore W2076789453C55493867 @default.
• W2076789453 hasConceptScore W2076789453C62520636 @default.
• W2076789453 hasConceptScore W2076789453C86803240 @default.
• W2076789453 hasConceptScore W2076789453C91881484 @default.
• W2076789453 hasIssue "13" @default.
• W2076789453 hasLocation W20767894531 @default.
• W2076789453 hasLocation W20767894532 @default.
• W2076789453 hasOpenAccess W2076789453 @default.

• next