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- W2076794741 abstract "Phage display is a widely used method to optimize the binding characteristics of protein-ligand interactions. In addition, it has been used to clone genes from genomic and cDNA libraries based on their ligand-binding characteristics. One difficulty often encountered when expressing heterologous proteins by phage display is the toxicity of the protein on the Escherichia coli host. Previous studies have shown that heterologous protein expression can be tightly controlled using plasmids with the P(BAD) promoter of the arabinose operon of E. coli, and the araC gene, which is both a positive and negative regulator of the promoter. We constructed a set of phage display vectors that utilize the P(BAD) promoter to control the expression of proteins on the surface of the M13 bacteriophage. These vectors exhibit tightly controlled expression of proteins on the surface of the phage. In addition, the amount of protein displayed on the phage is modulated by the amount of arabinose present in the growth medium during phage propagation. This may be useful for altering the stringency of binding enrichment during phage display." @default.
- W2076794741 created "2016-06-24" @default.
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- W2076794741 date "2000-06-01" @default.
- W2076794741 modified "2023-09-24" @default.
- W2076794741 title "Use of the arabinose pbad promoter for tightly regulated display of proteins on bacteriophage" @default.
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- W2076794741 doi "https://doi.org/10.1016/s0378-1119(00)00210-9" @default.
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