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- W2076799477 abstract "Fructosyltransferase activities were studied with varying concentrations of sucrose in cell-free extracts from wheat (Triticum aestivum L.). A C18-HPLC separation method was used to quantify the synthesized compounds individually. Hexoses and 1-kestose were the major products at all temperatures and concentrations of sucrose. 1-Kestose production was maximal at 25 °C, with an apparent Km of about 250 m, but competition for sucrose by invertase affected this value. Neokestose, which is not observed in vitro, and 6-kestose were also formed in vitro, but at a much lower rate and at lower sucrose affinity than 1-kestose synthesis. When extracts were incubated with a high initial concentration of sucrose for up to 1 d, five new fructan oligomers appeared. Four of these were also accumulated early in vitro by chilled blades, whereas the fifth belonged to the neokestose series. The rates of synthetic oligomers are compared to those in vivo. Invertase-linked artefacts are present in wheat, but not overwhelming as they are in Lolium temulentum." @default.
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- W2076799477 date "1993-07-01" @default.
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- W2076799477 title "Pattern of fructan synthesized in cell-free extracts from leaf blades of chilled Triticum aestivum" @default.
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- W2076799477 doi "https://doi.org/10.1111/j.1469-8137.1993.tb03827.x" @default.
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