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• W2076805557 abstract "Here we report on the feasibility of using replication deficient adenoviruses to modify signal transduction systems in epithelia. We constructed two viruses, one expressing a dominant negative mutant of the α-subunit of Gq (Ad-EF1-dnGαq) and the other expressing the wild-type α-subunit of Gq (Ad-EFi-wtGαq). We used an adenovirus expressing green fluorescent protein (Ad-EFi-GFP20) to show that infection of cultured cells with an adenovirus results in at least 95% expression of the transgene in both HSG and HT29 cells. We also used an adenovirus that expresses no transgene (Ad-MX17) to demonstrate that adenoviral infection itself does not affect the resting concentration of cytosolic Ca2+ ([Ca2+]i or the carbachol responses in these cells. We further show that Ad-EF1- dnGαq inhibits the increase in [Ca2+]i produced by muscarinic receptor activation in both the cell lines we studied. This inhibitory effect is not shared by Ad-EFi-wtGαq, which indicates that in both HSG and HT29 cells, the increase in [Ca 2+]i produced by muscarinic receptor activation is largely mediated by activation of Gq. Neither virus affected the resting level of [Ca2+]i in these cells. Our findings confirm the feasibility of using replication deficient adenoviruses expressing dominant negative mutants to investigate the role of G proteins in signal transduction systems." @default.
• W2076805557 created "2016-06-24" @default.
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• W2076805557 date "1998-08-01" @default.
• W2076805557 modified "2023-09-26" @default.
• W2076805557 title "Use of replication deficient adenoviruses to investigate the role of G proteins in Ca2+ signalling in epithelial cells" @default.
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• W2076805557 doi "https://doi.org/10.1016/s0143-4160(98)90077-x" @default.
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