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• W2076816475 abstract "To evaluate the utility of random amplification of polymorphic DNA (RAPD) technique for routine practice in public health laboratories for epidemiological studies of Vibrio cholerae O1 isolates.Seventy-nine strains were examined by PCR for the toxin genes (ctx A, zot and ace), virulence-associated genes (tcp A and tox T) and RAPD sequences. Except for one strain (no. 1123) from the Amazonas State, all the strains analysed carried the genes ctx A, zot, ace, tcp A and tox T. RAPD fingerprinting revealed variability but no correlation with serotype, biotype or geographical origin of the isolates was found.A standardized RAPD method does not enable the establishment of a pattern data bank for the identification of V. cholerae O1 strains.The simplicity and discriminative capacity of this technique make it useful for detecting genetic diversity among micro-organisms from a defined group or for outbreak investigation." @default.
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• W2076816475 date "2004-03-01" @default.
• W2076816475 modified "2023-09-26" @default.
• W2076816475 title "Evaluation of a RAPD-based typing scheme in a molecular epidemiology study of Vibrio cholerae O1, Brazil" @default.
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• W2076816475 doi "https://doi.org/10.1111/j.1365-2672.2004.02090.x" @default.
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