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- W2076850060 abstract "Infection with vaccinia mutant is 6757 under restrictive conditions results in formation of large quantities of viral envelopes. Unlike envelopes which normally surround immature virions, aberrant envelopes assembled under control of this mutant, designated IE, were formed apart from the internal constituents. However, like the envelopes of normal immature virions, IE possessed an external layer of spicules. Following isolation and concentration of IE, antisera were prepared in rabbits which specifically immuno-precipitated a major IE polypeptide of MW 65,000 labeled p65E. Experimental evidence was obtained by several approaches in identifying the spicules as the p65E polypeptide. Data obtained with antisera specific against p65E, combined with biochemical and electron-microscopic observations on the development of several ts mutants, including 6757, are consistent with the view that spicules attached onto envelopes function to provide transitory scaffolding. During virion maturation, the spicules are replaced by the surface tubular elements or STE, characterized by Stern and Dales ((1976), Virology, 75, 232–241)." @default.
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- W2076850060 date "1982-04-01" @default.
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- W2076850060 title "Biogenesis of vaccinia: Involvement of spicules of the envelope during virion assembly examined by means of conditional lethal mutants and serology" @default.
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- W2076850060 doi "https://doi.org/10.1016/0042-6822(82)90347-6" @default.
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