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• W2076854734 abstract "The repair activity of Escherichia coli DNA polymerase I (Klenow fragment) was used to prepare nonadecanucleotide hybridization probes which were complementary either to the normal human β-globin (βA) or to the sickle cell human β-globin (βS) gene. Template-directed polymerization of highly radiolabeled α[32P]deoxyribonucleoside triphosphates (dNTPs) onto nonamer and decamer primers produced probes with specific activities ranging from 1.0 × 1010 to 2.0 × 1010 dpm/μg. The extremely high specific activities of these probes made it possible to detect the βA and βS single-copy gene sequences in as little as 1 μg of total human genomic DNA as well as to discriminate between the homozygous and heterozygous states." @default.
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• W2076854734 date "1984-02-01" @default.
• W2076854734 modified "2023-10-14" @default.
• W2076854734 title "Allele-Specific Hybridization Using Oligonucleotide Probes of Very High Specific Activity: Discrimination of the Human β^A- and β^S-Globin Genes" @default.
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• W2076854734 doi "https://doi.org/10.1089/dna.1.1984.3.7" @default.
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