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- W2076876382 abstract "The human U1A protein has two putative RNA binding domains, one at the N-terminal region of the protein (RBD1) and the other at the C-terminal end (RBD2). RBD1 binds tightly and specifically to one of the stem loops of the U1 snRNA, as well as to its own 3‘-UTR. In contrast, RBD2 does not appear to associate with any RNA. The two domains share 25% amino acid identity, and both have the same βαβ−βαβ secondary structure fold. In this work, 13C/15N/1H multidimensional NMR methods were used to obtain side-chain assignments for RBD2, and then the tertiary structure was calculated using a distance geometry/simulated annealing algorithm that employs pairwise Gaussian metrization. RBD2 is shown to fold into an α/β sandwich with a four-stranded antiparallel β-sheet, which is the typical global topology of these domains. Specific structural features of RBD2 include a β-bulge in β2, N-capping boxes for both α-helices, and an extremely shallow twist of its β-sheet. The 15N backbone dynamics of these two structurally homologous RBDs are significantly different, compared using order parameters and T2 exchange terms in the Lipari and Szabo model-free formalism. Conformational exchange observed in RBD1, which is absent in RBD2, may correlate to the mechanism of RNA binding." @default.
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- W2076876382 date "1997-08-26" @default.
- W2076876382 modified "2023-09-26" @default.
- W2076876382 title "Tertiary Structure of RBD2 and Backbone Dynamics of RBD1 and RBD2 of the Human U1A Protein Determined by NMR Spectroscopy<sup>,</sup>" @default.
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- W2076876382 doi "https://doi.org/10.1021/bi9709811" @default.
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