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• W2076893265 abstract "Normal rat kidney (NRK) cells transformed by simian sarcoma virus (SSV) release into the culture medium a biologically active mitogen with properties identical to those of human platelet-derived growth factor (PDGF). Like PDGF, the growth factor derived from SSV-NRK cells was shown to be stable to heat and sensitive to reducing agents. It was capable of inhibiting binding of labeled PDGF to the receptor on human fibroblasts. It also stimulated the phosphorylation of the same membrane protein (185 kilodaltons) in isolated plasma membranes from human fibroblasts. Immunoprecipitation of metabolically labeled proteins released by SSV-NRK cells showed that a 34-kilodalton protein was specifically precipitated by antiserum to PDGF. Upon reduction, this protein had a molecular size of 17 kilodaltons. PDGF has been shown to consist of two 14- to 18-kilodalton proteins linked by disulfide bonds." @default.
• W2076893265 created "2016-06-24" @default.
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• W2076893265 date "1984-07-06" @default.
• W2076893265 modified "2023-10-16" @default.
• W2076893265 title "Simian Sarcoma Virus—Transformed Cells Secrete a Mitogen Identical to Platelet-Derived Growth Factor" @default.
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• W2076893265 doi "https://doi.org/10.1126/science.6328659" @default.
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