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• W2076918989 abstract "Primary cultures of astrocytes exhibit a polygonal morphology, but on treatment with agents that increase cAMP they change to stellate cells. We found that astrocyte stellation also occurred on replacing the culture medium with saline buffered with HEPES. However, stellation did not occur when the medium was replaced with saline buffered with bicarbonate/CO2 provided Ca2+ was present. Since exposure of astrocytes to media lacking bicarbonate results in a decrease in intracellular pH (pHi) we sought evidence for an association between pHi and morphology. Astrocytic pHi was monitored for 60 min after transferring the cells to HEPES or bicarbonate-buffered saline. HEPES-induced stellation was associated with transient acidification which coincided with the morphological changes. Acidification was not observed in cells transferred to bicarbonate–saline. However when cytoplasmic acidification of cells in bicarbonate–saline was induced pharmacologically, rapid stellation occurred. Stellation induced by cAMP is reversed by activation of the RhoA pathway with lysophosphatidic acid (LPA). Here we found that LPA inhibited HEPES-induced stellation, but only with Ca2+ present. Inhibition of stellation by LPA+Ca2+ was associated with transient acidification followed by modest alkanization. A close association of tyrosine phosphorylation with stellation and pHi was observed. Thus incubation of astrocytes in HEPES–saline with orthovanadate to inhibit dephosphorylation abolished stellation and acidification; conversely incubation of cells in bicarbonate–saline with genistein to inhibit tyrosine kinases caused stellation and major acidification. Acidification may be one of several factors resulting in stellation, but it is not a necessary factor since stellation without acidification was observed in bicarbonate–saline lacking Ca2+." @default.
• W2076918989 created "2016-06-24" @default.
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• W2076918989 date "2002-08-01" @default.
• W2076918989 modified "2023-10-14" @default.
• W2076918989 title "Astrocyte stellation in saline media lacking bicarbonate: possible relation to intracellular pH and tyrosine phosphorylation" @default.
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• W2076918989 doi "https://doi.org/10.1016/s0006-8993(02)02819-6" @default.
• W2076918989 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12133590" @default.
• W2076918989 hasPublicationYear "2002" @default.
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