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- W2076985250 abstract "Abstract Objectives of trial 1 were to delineate effects of glucose addition on digestion of cellulosic and hemicellulosic fiber sources in vitro as either pH-dependent or pH-independent. Two buffer media (regular and strong), three fiber sources (cellulose, soyhulls, and corn bran), and four glucose concentrations (0, 10, 25, and 50m M ) were used in an in vitro digestion system in a 2 × 3 × 4 factorial arrangement of treatments. Strong, but not regular, buffer medium maintained pH >6.2 when glucose was added. Strong buffer medium and 50m M glucose increased NDF digestion lag time. Digestion rate of cellulose was decreased when 25 or 50m M glucose was added compared with effects of no glucose in both buffer media. Digestion rate of soyhull NDF was decreased when 25 or 50m M glucose was added compared with no glucose in cultures containing regular, but not strong, buffer medium. Corn bran NDF digestion rate was decreased when 50m M glucose was added with strong, but not regular, buffer medium compared with effects of no glucose. Thus, effects of glucose addition on cellulose and corn bran digestion rates were, in part, pH-independent, but effect on soyhulls was pH-dependent. Effects of adding 25m M glucose at 0 or 24h of incubation in strong buffer medium on microbial colonization and digestion of fiber were studied in trial 2. Glucose added at 0h, but not at 24h, of incubation decreased NDF digestion rate compared with effects of no glucose. Glucose added at 0 or 24h of incubation decreased carboxymethylcellulase activity (index of colonization of fibrolytic microbes). Corn bran digesta had lower carboxymethylcellulase activity than did digesta from cellulose or soyhulls. Glucose decreased NDF digestion rate, apparently by decreasing colonization of fibrolytic microbes on cellulosic and hemicellulosic fiber sources." @default.
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- W2076985250 date "1993-01-01" @default.
- W2076985250 modified "2023-09-26" @default.
- W2076985250 title "Effect of Glucose on Fiber Digestion and Particle-Associated Carboxymethylcellulase Activity In Vitro" @default.
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- W2076985250 doi "https://doi.org/10.3168/jds.s0022-0302(93)77332-4" @default.
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