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- W2076989148 abstract "We have developed a simple and economical method for HLA-DNA typing, called microtiter plate hybridization (PCR-MPH), which could replace standard PCR-SSO. This method is similar to that of an ELISA. Briefly, the PCR products labeled at the 5′ termini with biotin were hybridized with probes immobilized on a microtiter well, and the bound PCR products were detected by streptavidin-conjugated enzymes followed by color development. A system for HLA-DRB1 “genetic” typing (e.g., DR1, DR2), using microtiter wells coated with 12 different SSOs has been established. The HLA-DRB types classified using this method agreed well with those obtained by conventional serologic typing. The advantages of this microtiter plate-hybridization method for routine HLA-DNA typing are a short assay time, easy processing of large numbers of samples, and the potential for automation." @default.
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- W2076989148 date "1994-10-01" @default.
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- W2076989148 title "A simple method of HLA-DRB typing using enzymatically amplified DNA and immobilized probes on microtiter plate" @default.
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- W2076989148 doi "https://doi.org/10.1016/0198-8859(94)90004-3" @default.
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