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- W2076993005 abstract "Betaine B (8-amino-5-ethyl-3-oxido-6-phenylphenanthridinium-betaine) is bound to DNA as protonated cation BH⊕ (8-amino-5-ethyl-3-hydroxy-6-phenylphenanthridinium-cation). BH⊕ is a hydroxy derivative of the ethidium cation E. Its binding to calf thymus DNA has been investigated by means of absorption spectroscopy. From binding isotherms we determined the binding constants of the non-competitive binding (intercalation) K1 = 0.9 · 104 M−1 and the competitive binding (external binding) K2 = 1.0 · 106 M−1 using NaCl as competitor, T = 298 K. The constant K1 of the intercalation is much more smaller than the constant K2 of the external binding. Similar properties has the ethidium cation E. – Unprotonated betaine B isn't bound to DNA. In a range of middle pH-values there exists an equilibrium in solution between the free unprotonated betaine B and the protonated bound betaine BH⊕. The free enthalpy of binding of BH⊕ to DNA forces the protonation of the base B." @default.
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- W2076993005 date "1982-08-01" @default.
- W2076993005 modified "2023-09-26" @default.
- W2076993005 title "Neuere Untersuchungen zur Bindung von Ethidiumbromid an DNA 4. Mitteilung: Betain B" @default.
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- W2076993005 doi "https://doi.org/10.1002/bbpc.19820860811" @default.
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