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• W2077007913 abstract "A hallmark of Alzheimer's disease (AD) brain is the amyloid β (Aβ) plaque, which is comprised of Aβ peptides. Multiple lines of evidence suggest that Aβ oligomers are more toxic than other peptide forms. We sought to develop a robust assay to quantify oligomers from CSF. Antibody 19.3 was compared in one-site and competitive ELISAs for oligomer binding specificity. A two-site ELISA for oligomers was developed using 19.3 coupled to a sensitive, bead-based fluorescent platform able to detect single photons of emitted light. The two-site ELISA was >2500× selective for Aβ oligomers over Aβ monomers with a limit of detection ∼ 0.09 pg/ml in human CSF. The lower limit of reliable quantification of the assay was 0.18 pg/ml and the antibody pairs recognized Aβ multimers comprised of either synthetic standards, or endogenous oligomers isolated from confirmed human AD and healthy control brain. Using the assay, a significant 3- to 5-fold increase in Aβ oligomers in human AD CSF compared with comparably aged controls was demonstrated. The increase was seen in three separate human cohorts, totaling 63 AD and 54 controls. CSF oligomers ranged between 0.1 and 10 pg/ml. Aβ oligomer levels did not strongly associate with age or gender, but had an inverse correlation with MMSE score. The C statistic for the Aβ oligomer ROC curve was 0.86, with 80% sensitivity and 88% specificity to detect AD, suggesting reasonable discriminatory power for the AD state and the potential for utility as a diagnostic marker." @default.
• W2077007913 created "2016-06-24" @default.
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• W2077007913 date "2014-02-19" @default.
• W2077007913 modified "2023-10-18" @default.
• W2077007913 title "A Sensitive A Oligomer Assay Discriminates Alzheimer's and Aged Control Cerebrospinal Fluid" @default.
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• W2077007913 cites W1964741226 @default.
• W2077007913 cites W1968740432 @default.
• W2077007913 cites W1970016175 @default.
• W2077007913 cites W1974145718 @default.
• W2077007913 cites W1982706802 @default.
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• W2077007913 cites W2006219887 @default.
• W2077007913 cites W2008301592 @default.
• W2077007913 cites W2013627371 @default.
• W2077007913 cites W2015746453 @default.
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• W2077007913 cites W2075481535 @default.
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• W2077007913 cites W2141854336 @default.
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• W2077007913 cites W2146411133 @default.
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• W2077007913 doi "https://doi.org/10.1523/jneurosci.1675-13.2014" @default.
• W2077007913 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6608513" @default.
• W2077007913 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24553930" @default.
• W2077007913 hasPublicationYear "2014" @default.
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