Matches in SemOpenAlex for { <https://semopenalex.org/work/W2077009168> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2077009168 endingPage "650" @default.
- W2077009168 startingPage "641" @default.
- W2077009168 abstract "1. Searching for endogenous proteolytic activities converting the membrane form of dopamine β-hydroxylase (dopamine β-monooxygenase, DBH) into the soluble and releasable form, DBH was monitored enzymatically and immunologically in aqueous and detergent-solubilized extracts of the adrenomedullary fractions. 2. Degradation of the soluble DBH and acidic chromogranins by activation of endogenous proteases occurred during lysis in H2O. 3. Shifts in the hydrophobicity of the membrane DBH were also apparent. Loss in enzyme protein or activity was, on the other hand, not observed for bufier-dialysed CG (pH 5–6). 4. Limited proteolysis within the membrane phase was, however, indicated by the shift towards dominance of the intermediate hydrophobic DBH in the buffer-dialysed CG. 5. By two-dimensional, crossed immunoelectrophoresis with cationic detergent the microsomal DBH was immunologically identical to the granule-bound enzyme but differed from the latter in molecular heterogeneity and in susceptibility to proteolytic solubilization by endogenous protease activities. 6. DBH in the membranes of the chromaffin granules was proteolytically solubilized at pH 6–8 and the soluble DBH further degraded at pH 5. 7. The results indicate that a post-translational conversion of the amphiphilic DBH into the soluble form, initiated at the level of the microsomes, may continue within the light and the heavy granule fractions which contain several DBH-converting and degrading proteolytic activities with acid optima." @default.
- W2077009168 created "2016-06-24" @default.
- W2077009168 creator A5010841081 @default.
- W2077009168 creator A5040308282 @default.
- W2077009168 creator A5041834895 @default.
- W2077009168 creator A5081728908 @default.
- W2077009168 date "1984-01-01" @default.
- W2077009168 modified "2023-09-25" @default.
- W2077009168 title "Membrane dopamine β-hydroxylase: a precursor for the soluble enzyme in the bovine adrenal medulla" @default.
- W2077009168 cites W1518330639 @default.
- W2077009168 cites W1964327588 @default.
- W2077009168 cites W1967105192 @default.
- W2077009168 cites W1971796313 @default.
- W2077009168 cites W1977485135 @default.
- W2077009168 cites W1981199893 @default.
- W2077009168 cites W1982227001 @default.
- W2077009168 cites W2002074814 @default.
- W2077009168 cites W2005592151 @default.
- W2077009168 cites W2011138241 @default.
- W2077009168 cites W2022110610 @default.
- W2077009168 cites W2034608686 @default.
- W2077009168 cites W2064736935 @default.
- W2077009168 cites W2084136509 @default.
- W2077009168 cites W2086650757 @default.
- W2077009168 cites W2088051799 @default.
- W2077009168 cites W2094678043 @default.
- W2077009168 cites W2139434603 @default.
- W2077009168 cites W2146923243 @default.
- W2077009168 cites W2154984407 @default.
- W2077009168 cites W2175970545 @default.
- W2077009168 cites W2411005849 @default.
- W2077009168 doi "https://doi.org/10.1016/0020-711x(84)90033-8" @default.
- W2077009168 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6468729" @default.
- W2077009168 hasPublicationYear "1984" @default.
- W2077009168 type Work @default.
- W2077009168 sameAs 2077009168 @default.
- W2077009168 citedByCount "10" @default.
- W2077009168 crossrefType "journal-article" @default.
- W2077009168 hasAuthorship W2077009168A5010841081 @default.
- W2077009168 hasAuthorship W2077009168A5040308282 @default.
- W2077009168 hasAuthorship W2077009168A5041834895 @default.
- W2077009168 hasAuthorship W2077009168A5081728908 @default.
- W2077009168 hasConcept C169760540 @default.
- W2077009168 hasConcept C181199279 @default.
- W2077009168 hasConcept C182220744 @default.
- W2077009168 hasConcept C185592680 @default.
- W2077009168 hasConcept C2775894120 @default.
- W2077009168 hasConcept C2776714187 @default.
- W2077009168 hasConcept C2777597313 @default.
- W2077009168 hasConcept C2781307694 @default.
- W2077009168 hasConcept C55493867 @default.
- W2077009168 hasConcept C57409179 @default.
- W2077009168 hasConcept C86803240 @default.
- W2077009168 hasConceptScore W2077009168C169760540 @default.
- W2077009168 hasConceptScore W2077009168C181199279 @default.
- W2077009168 hasConceptScore W2077009168C182220744 @default.
- W2077009168 hasConceptScore W2077009168C185592680 @default.
- W2077009168 hasConceptScore W2077009168C2775894120 @default.
- W2077009168 hasConceptScore W2077009168C2776714187 @default.
- W2077009168 hasConceptScore W2077009168C2777597313 @default.
- W2077009168 hasConceptScore W2077009168C2781307694 @default.
- W2077009168 hasConceptScore W2077009168C55493867 @default.
- W2077009168 hasConceptScore W2077009168C57409179 @default.
- W2077009168 hasConceptScore W2077009168C86803240 @default.
- W2077009168 hasIssue "6" @default.
- W2077009168 hasLocation W20770091681 @default.
- W2077009168 hasLocation W20770091682 @default.
- W2077009168 hasOpenAccess W2077009168 @default.
- W2077009168 hasPrimaryLocation W20770091681 @default.
- W2077009168 hasRelatedWork W1220851060 @default.
- W2077009168 hasRelatedWork W17428494 @default.
- W2077009168 hasRelatedWork W1999929305 @default.
- W2077009168 hasRelatedWork W2491451478 @default.
- W2077009168 hasRelatedWork W2531675153 @default.
- W2077009168 hasRelatedWork W2912440625 @default.
- W2077009168 hasRelatedWork W3191591153 @default.
- W2077009168 hasRelatedWork W4280491523 @default.
- W2077009168 hasRelatedWork W4386271821 @default.
- W2077009168 hasRelatedWork W44373803 @default.
- W2077009168 hasVolume "16" @default.
- W2077009168 isParatext "false" @default.
- W2077009168 isRetracted "false" @default.
- W2077009168 magId "2077009168" @default.
- W2077009168 workType "article" @default.