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- W2077029450 abstract "Cell disruption studies for the extraction of HBsAg from a recombinant P. pastoris strain (r-HBsAg) were done using a bead mill disintegrator. Three sequential passages (4 min retention time each) were enough to disrupt the cells and extract most of the r-HBsAg and soluble proteins. An acid precipitation step was performed just after cell disruption to precipitate proteins together with the cell debris. Different precipitation pH values (2.5 to 6.0) were investigated. A pH value of 4.2 was selected as a compromise between recovery and improvement of specific activity. A 6 to 8-fold enhancement of the specific activity was obtained, having a r-HBsAg overall yield of about 80%. The influencing presence of a chaotropic salt (potassium thiocyanate) during the acid precipitation step was also studied." @default.
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- W2077029450 date "1993-01-01" @default.
- W2077029450 modified "2023-09-27" @default.
- W2077029450 title "The recovery of the hepatitis B virus surface antigen (HBsAg) from a recombinantP. pastorisstrain disruption and precipitation studies" @default.
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- W2077029450 doi "https://doi.org/10.1002/abio.370130207" @default.
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