FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2077033545> ?p ?o ?g. }

• W2077033545 endingPage "72" @default.
• W2077033545 startingPage "65" @default.
• W2077033545 abstract "Osteoarthritis (OA) is the most common form of arthritis and patients with meniscal and ligament injuries of the knee are at high risk to develop the disease. The purpose of this study was to evaluate changes occurring in both medial and lateral menisci from the knees of anterior cruciate ligament (ACL) transected rabbits at 3 and 8 weeks post-surgery. This study describes both molecular and cellular alterations in menisci during the early stages of OA development.Rabbit meniscal tissues were processed for molecular analysis: DNA and RNA concentrations were assessed, as well as semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) analysis for a subset of relevant molecules was performed. In situ DNA fragmentation was evaluated using the TUNEL assay.Total RNA yields from the medial meniscus were significantly elevated at both 3 and 8 weeks post-ACL transection, while in the lateral meniscus total RNA levels were unchanged following ACL transection. DNA concentrations were significantly decreased in the medial menisci only at 8 weeks post-ACL transection. Following ACL transection, analysis of in situ DNA fragmentation using the TUNEL assay demonstrated an increase in the number of apoptotic cells in the medial meniscus only, in particular at 3 weeks post-ACL transection, a finding which correlates with declines in DNA content. Analysis of specific mRNA levels by RT-PCR demonstrated complex changes in both menisci following ACL transection. At 3 and 8 weeks post-ACL transection, in both medial and lateral menisci, mRNA levels for type I collagen and TIMP-1 were significantly increased, while mRNA levels for decorin, TNF-alpha and IGF-2 were significantly depressed. In the medial meniscus, significant increases in mRNA levels for type II collagen, biglycan as well as iNOS and PAI-1 were detected at both time periods, while mRNA levels for aggrecan, type III collagen and COX-2 were significantly elevated at 3 weeks post-ACL transection and mRNA levels for MMP-1 were significantly elevated at 8 weeks post-ACL transection. In contrast, mRNA levels for COL2 and aggrecan were unchanged in the lateral meniscus following ACL transection. In the lateral meniscus, at 3 weeks post-ACL transection, type III collagen mRNA levels were dramatically increased while fibromodulin mRNA levels were significantly depressed. In the lateral meniscus, significant increases in mRNA levels for biglycan were detected at 8 weeks post-ACL transection.These results show that after ACL transection complex molecular changes, as well as apoptosis, occur early, particularly in the medial meniscus." @default.
• W2077033545 created "2016-06-24" @default.
• W2077033545 creator A5027703531 @default.
• W2077033545 creator A5044951935 @default.
• W2077033545 creator A5061396807 @default.
• W2077033545 creator A5085084161 @default.
• W2077033545 date "2001-01-01" @default.
• W2077033545 modified "2023-10-16" @default.
• W2077033545 title "Early changes in lapine menisci during osteoarthritis development Part II: Molecular alterations" @default.
• W2077033545 cites W1527619287 @default.
• W2077033545 cites W1967521496 @default.
• W2077033545 cites W1968422024 @default.
• W2077033545 cites W1977253236 @default.
• W2077033545 cites W1983835698 @default.
• W2077033545 cites W1986317985 @default.
• W2077033545 cites W1987188469 @default.
• W2077033545 cites W2002583834 @default.
• W2077033545 cites W2024217515 @default.
• W2077033545 cites W2039587194 @default.
• W2077033545 cites W2052866874 @default.
• W2077033545 cites W2079568970 @default.
• W2077033545 cites W2083784930 @default.
• W2077033545 cites W2087870230 @default.
• W2077033545 cites W2094637500 @default.
• W2077033545 cites W2117983863 @default.
• W2077033545 cites W2130415027 @default.
• W2077033545 cites W2144245646 @default.
• W2077033545 cites W4253061146 @default.
• W2077033545 doi "https://doi.org/10.1053/joca.2000.0351" @default.
• W2077033545 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11178949" @default.
• W2077033545 hasPublicationYear "2001" @default.
• W2077033545 type Work @default.
• W2077033545 sameAs 2077033545 @default.
• W2077033545 citedByCount "81" @default.
• W2077033545 countsByYear W20770335452012 @default.
• W2077033545 countsByYear W20770335452013 @default.
• W2077033545 countsByYear W20770335452014 @default.
• W2077033545 countsByYear W20770335452015 @default.
• W2077033545 countsByYear W20770335452016 @default.
• W2077033545 countsByYear W20770335452017 @default.
• W2077033545 countsByYear W20770335452018 @default.
• W2077033545 countsByYear W20770335452019 @default.
• W2077033545 countsByYear W20770335452020 @default.
• W2077033545 countsByYear W20770335452021 @default.
• W2077033545 countsByYear W20770335452023 @default.
• W2077033545 crossrefType "journal-article" @default.
• W2077033545 hasAuthorship W2077033545A5027703531 @default.
• W2077033545 hasAuthorship W2077033545A5044951935 @default.
• W2077033545 hasAuthorship W2077033545A5061396807 @default.
• W2077033545 hasAuthorship W2077033545A5085084161 @default.
• W2077033545 hasBestOaLocation W20770335451 @default.
• W2077033545 hasConcept C104317684 @default.
• W2077033545 hasConcept C105580179 @default.
• W2077033545 hasConcept C105702510 @default.
• W2077033545 hasConcept C120665830 @default.
• W2077033545 hasConcept C121332964 @default.
• W2077033545 hasConcept C142724271 @default.
• W2077033545 hasConcept C185592680 @default.
• W2077033545 hasConcept C18903297 @default.
• W2077033545 hasConcept C189178095 @default.
• W2077033545 hasConcept C190283241 @default.
• W2077033545 hasConcept C191015642 @default.
• W2077033545 hasConcept C196795494 @default.
• W2077033545 hasConcept C204232928 @default.
• W2077033545 hasConcept C204787440 @default.
• W2077033545 hasConcept C2776164576 @default.
• W2077033545 hasConcept C2778434673 @default.
• W2077033545 hasConcept C2779699715 @default.
• W2077033545 hasConcept C2779753412 @default.
• W2077033545 hasConcept C2780423099 @default.
• W2077033545 hasConcept C2781170992 @default.
• W2077033545 hasConcept C31573885 @default.
• W2077033545 hasConcept C43759708 @default.
• W2077033545 hasConcept C48023723 @default.
• W2077033545 hasConcept C55493867 @default.
• W2077033545 hasConcept C61511704 @default.
• W2077033545 hasConcept C71924100 @default.
• W2077033545 hasConcept C81439078 @default.
• W2077033545 hasConcept C86803240 @default.
• W2077033545 hasConceptScore W2077033545C104317684 @default.
• W2077033545 hasConceptScore W2077033545C105580179 @default.
• W2077033545 hasConceptScore W2077033545C105702510 @default.
• W2077033545 hasConceptScore W2077033545C120665830 @default.
• W2077033545 hasConceptScore W2077033545C121332964 @default.
• W2077033545 hasConceptScore W2077033545C142724271 @default.
• W2077033545 hasConceptScore W2077033545C185592680 @default.
• W2077033545 hasConceptScore W2077033545C18903297 @default.
• W2077033545 hasConceptScore W2077033545C189178095 @default.
• W2077033545 hasConceptScore W2077033545C190283241 @default.
• W2077033545 hasConceptScore W2077033545C191015642 @default.
• W2077033545 hasConceptScore W2077033545C196795494 @default.
• W2077033545 hasConceptScore W2077033545C204232928 @default.
• W2077033545 hasConceptScore W2077033545C204787440 @default.
• W2077033545 hasConceptScore W2077033545C2776164576 @default.
• W2077033545 hasConceptScore W2077033545C2778434673 @default.
• W2077033545 hasConceptScore W2077033545C2779699715 @default.
• W2077033545 hasConceptScore W2077033545C2779753412 @default.
• W2077033545 hasConceptScore W2077033545C2780423099 @default.

• next