FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2077074145> ?p ?o ?g. }

• W2077074145 endingPage "242" @default.
• W2077074145 startingPage "235" @default.
• W2077074145 abstract "Thanatin(S) is an analog of thanatin, an insect antimicrobial peptide possessing strong and broad spectrum of antimicrobial activity. In order to investigate if the thanatin could be used in engineering transgenic plants for increased resistance against phytopathogens, the synthetic thanatin(S) was introduced into Arabidopsis thaliana plants. To increase the expression level of thanatin(S) in plants, the coding sequence was optimized by plant-preference codon. To avoid cellular protease degradation, signal peptide of rice Cht1 was fused to N terminal of thanatin(S) for secreting the expressed thanatin(S) into intercellular spaces. To evaluate the application value of thanatin(S) in plant disease control, the synthesized coding sequence of Cht1 signal peptide (Cht1SP)-thanatin(S) was ligated to plant gateway destination binary vectors pGWB11 (with FLAG tag). Meanwhile, in order to observe the subcellular localization of Cht1SP-thanatin(S)-GFP and thanatin(S)-GFP, the sequences of Cht1SP-thanatin(S) and thanatin(S) were respectively linked to pGWB5 (with GFP tag). The constructs were transformed into Arabidopsis ecotype Col-0 and mutant pad4-1 via Agrobacterium-mediated transformation. The transformants with Cht1SP-thanatin(S)-FLAG fusion gene were analyzed by genomic PCR, real-time PCR, and western blots and the transgenic Arabidopsis plants introduced respectively Cht1SP-thanatin(S)-GFP and thanatin(S)-GFP were observed by confocal microscopy. Transgenic plants expressing Cht1SP-thanatin(S)-FLAG fusion protein showed antifungal activity against Botrytis cinerea and powdery mildew, as well as antibacterial activity against Pseudomonas syringae pv. tomato. And the results from confocal observation showed that the GFP signal from Cht1SP-thanatin(S)-GFP transgenic Arabidopsis plants occurred mainly in intercellular space, while that from thanatin(S)-GFP transgenic plants was mainly detected in the cytoplasm and that from empty vector transgenic plants was distributed uniformly throughout the cell, demonstrating that Cht1 signal peptide functioned. In addition, thanatin(S) and thanatin(S)-FLAG chemically synthesized have both in vitro antimicrobial activities against P. syringae pv. tomato and B. cinerea. So, thanatin(S) is an ideal candidate AMPs for the construction of transgenic crops endowed with a broad-spectrum resistance to phytopathogens and the strategy is feasible to link a signal peptide to the target gene." @default.
• W2077074145 created "2016-06-24" @default.
• W2077074145 creator A5017448268 @default.
• W2077074145 creator A5017473163 @default.
• W2077074145 creator A5023872784 @default.
• W2077074145 creator A5027695074 @default.
• W2077074145 creator A5033247500 @default.
• W2077074145 creator A5062660907 @default.
• W2077074145 date "2013-09-01" @default.
• W2077074145 modified "2023-10-02" @default.
• W2077074145 title "Expression of antimicrobial peptides thanatin(S) in transgenic Arabidopsis enhanced resistance to phytopathogenic fungi and bacteria" @default.
• W2077074145 cites W1514282028 @default.
• W2077074145 cites W1533287645 @default.
• W2077074145 cites W1566117045 @default.
• W2077074145 cites W1884528635 @default.
• W2077074145 cites W1932988598 @default.
• W2077074145 cites W1981646808 @default.
• W2077074145 cites W1999930139 @default.
• W2077074145 cites W2000353148 @default.
• W2077074145 cites W2009012876 @default.
• W2077074145 cites W2010837704 @default.
• W2077074145 cites W2025240314 @default.
• W2077074145 cites W2032437941 @default.
• W2077074145 cites W2039764190 @default.
• W2077074145 cites W2043713622 @default.
• W2077074145 cites W2055155126 @default.
• W2077074145 cites W2060519093 @default.
• W2077074145 cites W2075343750 @default.
• W2077074145 cites W2075370926 @default.
• W2077074145 cites W2081408539 @default.
• W2077074145 cites W2082091267 @default.
• W2077074145 cites W2091027292 @default.
• W2077074145 cites W2091057420 @default.
• W2077074145 cites W2094662908 @default.
• W2077074145 cites W2101064142 @default.
• W2077074145 cites W2116122104 @default.
• W2077074145 cites W2133554515 @default.
• W2077074145 cites W2161129756 @default.
• W2077074145 cites W2166991901 @default.
• W2077074145 cites W2169768163 @default.
• W2077074145 doi "https://doi.org/10.1016/j.gene.2013.06.037" @default.
• W2077074145 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23820081" @default.
• W2077074145 hasPublicationYear "2013" @default.
• W2077074145 type Work @default.
• W2077074145 sameAs 2077074145 @default.
• W2077074145 citedByCount "24" @default.
• W2077074145 countsByYear W20770741452014 @default.
• W2077074145 countsByYear W20770741452015 @default.
• W2077074145 countsByYear W20770741452016 @default.
• W2077074145 countsByYear W20770741452017 @default.
• W2077074145 countsByYear W20770741452018 @default.
• W2077074145 countsByYear W20770741452019 @default.
• W2077074145 countsByYear W20770741452020 @default.
• W2077074145 countsByYear W20770741452021 @default.
• W2077074145 countsByYear W20770741452022 @default.
• W2077074145 countsByYear W20770741452023 @default.
• W2077074145 crossrefType "journal-article" @default.
• W2077074145 hasAuthorship W2077074145A5017448268 @default.
• W2077074145 hasAuthorship W2077074145A5017473163 @default.
• W2077074145 hasAuthorship W2077074145A5023872784 @default.
• W2077074145 hasAuthorship W2077074145A5027695074 @default.
• W2077074145 hasAuthorship W2077074145A5033247500 @default.
• W2077074145 hasAuthorship W2077074145A5062660907 @default.
• W2077074145 hasConcept C102230213 @default.
• W2077074145 hasConcept C104317684 @default.
• W2077074145 hasConcept C122172344 @default.
• W2077074145 hasConcept C143065580 @default.
• W2077074145 hasConcept C2776460866 @default.
• W2077074145 hasConcept C2779491563 @default.
• W2077074145 hasConcept C2780381237 @default.
• W2077074145 hasConcept C54355233 @default.
• W2077074145 hasConcept C86803240 @default.
• W2077074145 hasConcept C89423630 @default.
• W2077074145 hasConceptScore W2077074145C102230213 @default.
• W2077074145 hasConceptScore W2077074145C104317684 @default.
• W2077074145 hasConceptScore W2077074145C122172344 @default.
• W2077074145 hasConceptScore W2077074145C143065580 @default.
• W2077074145 hasConceptScore W2077074145C2776460866 @default.
• W2077074145 hasConceptScore W2077074145C2779491563 @default.
• W2077074145 hasConceptScore W2077074145C2780381237 @default.
• W2077074145 hasConceptScore W2077074145C54355233 @default.
• W2077074145 hasConceptScore W2077074145C86803240 @default.
• W2077074145 hasConceptScore W2077074145C89423630 @default.
• W2077074145 hasIssue "1" @default.
• W2077074145 hasLocation W20770741451 @default.
• W2077074145 hasLocation W20770741452 @default.
• W2077074145 hasOpenAccess W2077074145 @default.
• W2077074145 hasPrimaryLocation W20770741451 @default.
• W2077074145 hasRelatedWork W1994569530 @default.
• W2077074145 hasRelatedWork W1998377076 @default.
• W2077074145 hasRelatedWork W2079809184 @default.
• W2077074145 hasRelatedWork W2107105264 @default.
• W2077074145 hasRelatedWork W2117338545 @default.
• W2077074145 hasRelatedWork W2131547186 @default.
• W2077074145 hasRelatedWork W2809848277 @default.
• W2077074145 hasRelatedWork W2903358884 @default.
• W2077074145 hasRelatedWork W4213453719 @default.
• W2077074145 hasRelatedWork W4236645060 @default.

• next