FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2077087730> ?p ?o ?g. }

• W2077087730 endingPage "1062" @default.
• W2077087730 startingPage "1053" @default.
• W2077087730 abstract "Primary cultures of cerebellar granule cells, prepared from cerebella of 7-day-old rats and cultured for 4 or 8 days, were used to study the neurodifferentiative effect of a GABAA receptor agonist, 4,5,6,7-tetrahydroisoxazol[5,4-c]pyridin-3-ol (THIP), on the expression of the α6 GABAA receptor subunit. Membranes prepared from these cultures were photolabeled with the imidazobenzodiazepine [3H]Ro15-4513. In THIP-treated cultures at 4 days in vitro (DIV), photolabeled [3H]Ro15-4513 binding in membranes was significantly increased for both the 51 kilodalton, kDa, (α1 subunit) and 56-kDa (α6 subunit) radioactive peaks in sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). In contrast, THIP-treated granule cells at 8 DIV demonstrated a small but significant decrease from control cultures in the photoincorporation of [3H]Ro15-4513 in the 51-kDa peak; however, no significant change in [3H]Ro15-4513 binding was observed for the 56-kDa polypeptide. Immunolabeling of the α6 subunit using silver-enhanced, immuno-gold staining of granule cells showed a significant effect with THIP treatment only at 4 DIV and not at 8 DIV. Examination by light microscopy demonstrated that the major effect of THIP was to increase α6 subunit clustering on granule cell bodies as well as neurites, 15-fold and sixfold, respectively. Using in situ hybridization, a small THIP-induced increase in α6 mRNA was detected at 4 DIV; however, no effect was apparent at 8 DIV. These data suggest that THIP has a trophic effect on α6 subunit expression, and this effect occurs only at an early developmental stage. Moreover, this study presents further evidence for the role of GABAA agonists, and thus the neurotransmitter, GABA, in regulating the expression of GABAA receptor subunits in the developing cerebellum. J. Neurosci. Res. 50:1053–1062, 1997. © 1997 Wiley-Liss, Inc." @default.
• W2077087730 created "2016-06-24" @default.
• W2077087730 creator A5008019188 @default.
• W2077087730 creator A5032973398 @default.
• W2077087730 creator A5052163444 @default.
• W2077087730 creator A5060318273 @default.
• W2077087730 creator A5060434388 @default.
• W2077087730 creator A5066912472 @default.
• W2077087730 date "1997-12-15" @default.
• W2077087730 modified "2023-09-25" @default.
• W2077087730 title "Expression of the GABAA receptor ?6 subunit in cultured cerebellar granule cells is developmentally regulated by activation of GABAA receptors" @default.
• W2077087730 cites W1504992746 @default.
• W2077087730 cites W1524203275 @default.
• W2077087730 cites W1551663317 @default.
• W2077087730 cites W1606910143 @default.
• W2077087730 cites W1994831700 @default.
• W2077087730 cites W1997257246 @default.
• W2077087730 cites W2001435643 @default.
• W2077087730 cites W2006156983 @default.
• W2077087730 cites W2008344134 @default.
• W2077087730 cites W2019923712 @default.
• W2077087730 cites W2023546249 @default.
• W2077087730 cites W2027883835 @default.
• W2077087730 cites W2028092195 @default.
• W2077087730 cites W2033874647 @default.
• W2077087730 cites W2039982472 @default.
• W2077087730 cites W2060586131 @default.
• W2077087730 cites W2061920760 @default.
• W2077087730 cites W2065775311 @default.
• W2077087730 cites W2065878109 @default.
• W2077087730 cites W2078319476 @default.
• W2077087730 cites W2078418843 @default.
• W2077087730 cites W2129151401 @default.
• W2077087730 cites W2164412530 @default.
• W2077087730 cites W2176076159 @default.
• W2077087730 cites W2652000709 @default.
• W2077087730 cites W4317922783 @default.
• W2077087730 cites W77926042 @default.
• W2077087730 doi "https://doi.org/10.1002/(sici)1097-4547(19971215)50:6<1053::aid-jnr17>3.0.co;2-5" @default.
• W2077087730 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9452021" @default.
• W2077087730 hasPublicationYear "1997" @default.
• W2077087730 type Work @default.
• W2077087730 sameAs 2077087730 @default.
• W2077087730 citedByCount "15" @default.
• W2077087730 countsByYear W20770877302013 @default.
• W2077087730 countsByYear W20770877302022 @default.
• W2077087730 crossrefType "journal-article" @default.
• W2077087730 hasAuthorship W2077087730A5008019188 @default.
• W2077087730 hasAuthorship W2077087730A5032973398 @default.
• W2077087730 hasAuthorship W2077087730A5052163444 @default.
• W2077087730 hasAuthorship W2077087730A5060318273 @default.
• W2077087730 hasAuthorship W2077087730A5060434388 @default.
• W2077087730 hasAuthorship W2077087730A5066912472 @default.
• W2077087730 hasConcept C104292427 @default.
• W2077087730 hasConcept C104317684 @default.
• W2077087730 hasConcept C134018914 @default.
• W2077087730 hasConcept C153911025 @default.
• W2077087730 hasConcept C168258287 @default.
• W2077087730 hasConcept C170493617 @default.
• W2077087730 hasConcept C185592680 @default.
• W2077087730 hasConcept C2776464000 @default.
• W2077087730 hasConcept C2778888523 @default.
• W2077087730 hasConcept C2778938600 @default.
• W2077087730 hasConcept C2779652256 @default.
• W2077087730 hasConcept C529278444 @default.
• W2077087730 hasConcept C55493867 @default.
• W2077087730 hasConcept C86803240 @default.
• W2077087730 hasConcept C95444343 @default.
• W2077087730 hasConceptScore W2077087730C104292427 @default.
• W2077087730 hasConceptScore W2077087730C104317684 @default.
• W2077087730 hasConceptScore W2077087730C134018914 @default.
• W2077087730 hasConceptScore W2077087730C153911025 @default.
• W2077087730 hasConceptScore W2077087730C168258287 @default.
• W2077087730 hasConceptScore W2077087730C170493617 @default.
• W2077087730 hasConceptScore W2077087730C185592680 @default.
• W2077087730 hasConceptScore W2077087730C2776464000 @default.
• W2077087730 hasConceptScore W2077087730C2778888523 @default.
• W2077087730 hasConceptScore W2077087730C2778938600 @default.
• W2077087730 hasConceptScore W2077087730C2779652256 @default.
• W2077087730 hasConceptScore W2077087730C529278444 @default.
• W2077087730 hasConceptScore W2077087730C55493867 @default.
• W2077087730 hasConceptScore W2077087730C86803240 @default.
• W2077087730 hasConceptScore W2077087730C95444343 @default.
• W2077087730 hasIssue "6" @default.
• W2077087730 hasLocation W20770877301 @default.
• W2077087730 hasLocation W20770877302 @default.
• W2077087730 hasOpenAccess W2077087730 @default.
• W2077087730 hasPrimaryLocation W20770877301 @default.
• W2077087730 hasRelatedWork W1493326255 @default.
• W2077087730 hasRelatedWork W1971014454 @default.
• W2077087730 hasRelatedWork W1973900806 @default.
• W2077087730 hasRelatedWork W2008441940 @default.
• W2077087730 hasRelatedWork W2032717282 @default.
• W2077087730 hasRelatedWork W2045237222 @default.
• W2077087730 hasRelatedWork W2064012910 @default.
• W2077087730 hasRelatedWork W2075638799 @default.
• W2077087730 hasRelatedWork W2076106945 @default.
• W2077087730 hasRelatedWork W2129114430 @default.

• next