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- W2077112968 abstract "We have constructed an arrayed, large insert, multiple coverage genomic library of Pneumocystis carinii DNA using the bacteriophage P1 cloning system. The library consists of ∽4800 independent clones with an average insert size of ∽55 kbp individually arrayed in 50 microtiter plates, and is readily screened on ten or fewer microtiter plate-sized filters using a high density colony replicating device. Screening of the library for unique P. carinii sequences detected an average of 4–5 positive clones for each, consistent with a several-fold coverage of the ∽10-mbp P. carinii genome. Restriction and hybridization analyses demonstrated that the P1 clones in this library are quite stable and contain few, if any, chimeric inserts. Thus, this arrayed, large insert library off. carinii genomic DNA will be a valuable tool in the future genetic dissection of this important pathogen." @default.
- W2077112968 created "2016-06-24" @default.
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- W2077112968 date "1996-05-01" @default.
- W2077112968 modified "2023-09-27" @default.
- W2077112968 title "An Arrayed Bacteriophage P1 Genomic Library of Pneumocystis carinii" @default.
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- W2077112968 doi "https://doi.org/10.1111/j.1550-7408.1996.tb01386.x" @default.
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