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- W2077143268 abstract "Abstract The enzymes, pancreatic RNAase and/or pronase, are often used as part of procedures for preparation of DNA of high molecular weight. However, we have found that the techniques commonly employed to inactivate contaminating DNA nucleases are not sufficient. We have demonstrated by means of agarose-acrylamide gel electrophoresis analysis of a mixture of RNA and DNA that repeated heating to 100°C for 10 min is not sufficient to inactivate DNA nucleases present in some commercial preparations of pancreatic RNAase dissolved at 2 mg/ml in distilled water. Similarly; we showed that allowing a 1 mg/ml solution of pronase to self-digest for 2 h at 37°C is not sufficient to rid it of contaminating DNA nucleases." @default.
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- W2077143268 date "1974-05-01" @default.
- W2077143268 modified "2023-09-25" @default.
- W2077143268 title "Problems in the extraction of DNA when utilizing pancreatic RNAase and pronase" @default.
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- W2077143268 doi "https://doi.org/10.1016/0005-2787(74)90075-6" @default.
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