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- W2077174342 abstract "A novel heat shock protein 70 (HSP70) gene, pss1+, of fission yeast, Schizosaccharomyces pombe (S. pombe), has been isolated as a multicopy suppressor of a synthetic lethal mutant of ras1+, which shows severe retardation of growth and aggregation phenotype when the ras1 gene function is absent. The pss1+ gene functionally complements the growth defect of the mutant. Sequence analysis revealed that pss1+ encodes an open reading frame (ORF) of 730 amino acids that is homologous to the HSP70 family proteins. The Pss1 has high homology to the Saccharomyces cerevisiae (S. cerevisiae) heat shock protein Sse1p/Msi3p (43% identity) that belongs to the HSP110/SSE subfamily of HSP70. The consensus nucleotide sequence of the heat shock element (HSE) was found in the upstream region of pss1+ gene. The transcript level of pss1+ was moderately abundant during steady-state growth at 25°C and increased a few-fold upon shifting to 42°C. Furthermore, transcription of pss1+ increased in nitrogen-starved conditions. Disruption of the pss1+ gene confers a temperature-sensitive growth phenotype and unexpectedly causes the increase in thermotolerance in S. pombe." @default.
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- W2077174342 date "1998-03-01" @default.
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- W2077174342 title "Isolation of a novel heat shock protein 70-like gene, pss1+ of Schizosaccharomyces pombe homologous to hsp110/SSE subfamily" @default.
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- W2077174342 doi "https://doi.org/10.1016/s0378-1119(98)00061-4" @default.
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