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• W2077183724 endingPage "1682" @default.
• W2077183724 startingPage "1671" @default.
• W2077183724 abstract "Reactive gliosis is a prominent result of many types of insult to the central nervous system (CNS) and leads to the formation of glial scar that impedes the regeneration of axons. The intermediate filament protein vimentin is found in pathology of the CNS, mainly in the vicinity of injuries to the CNS. In the present study we investigated the role of vimentin in the formation of glial scars in vitro and in vivo by using immunohistochemistry, Western blot analysis, and in situ hybridization. In vitro experiments showed that the intensity of immunofluorescent labeling for vimentin and glial fibrillary acidic protein (GFAP) was consistently decreased in astrocytes after transfection with a retrovirus carrying antisense complementary DNA (cDNA) for vimentin. Transfection also inhibited the growth of astrocytes and decreased the expression of vimentin mRNA. In vivo studies demonstrated that transfection with the retrovirus carrying the antisense cDNA vimentin inhibited the upregulation of vimentin and GFAP in stab wounds in rat cerebrum. These results suggest that vimentin may play a key role in the formation of glial scars in the CNS." @default.
• W2077183724 created "2016-06-24" @default.
• W2077183724 creator A5021149358 @default.
• W2077183724 creator A5027188936 @default.
• W2077183724 date "2004-11-01" @default.
• W2077183724 modified "2023-09-23" @default.
• W2077183724 title "Effect of Vimentin on Reactive Gliosis:<i>In Vitro</i>and<i>In Vivo</i>Analysis" @default.
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• W2077183724 doi "https://doi.org/10.1089/neu.2004.21.1671" @default.
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