FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2077189905> ?p ?o ?g. }

• W2077189905 abstract "Abstract Background Retroviral integrases (INs) catalyze the integration of viral DNA in the chromosomal DNA of the infected cell. This reaction requires the multimerization of IN to coordinate a nucleophilic attack of the 3’ ends of viral DNA at two staggered phosphodiester bonds on the recipient DNA. Several models indicate that a tetramer of IN would be required for two-end concerted integration. Complementation assays have shown that the N-terminal domain (NTD) of integrase is essential for concerted integration, contributing to the formation of a multimer through protein-protein interaction. The isolated NTD of Mo-MLV integrase behave as a dimer in solution however the structure of the dimer in solution is not known. Results In this work, crosslinking and mass spectrometry were used to identify regions involved in the dimerization of the isolated Mo-MLV NTD. The distances between the crosslinked lysines within the monomer are in agreement with the structure of the NTD monomer found in 3NNQ. The intermolecular crosslinked peptides corresponding to Lys 20-Lys 31, Lys 24-Lys 24 and Lys 68-Lys 88 were identified. The 3D coordinates of 3NNQ were used to derive a theoretical structure of the NTD dimer with the suite 3D-Dock, based on shape and electrostatics complementarity, and filtered with the distance restraints determined in the crosslinking experiments. Conclusions The crosslinking results are consistent with the monomeric structure of NTD in 3NNQ, but for the dimer, in our model both polypeptides are oriented in parallel with each other and the contacting areas between the monomers would involve the interactions between helices 1 and helices 3 and 4." @default.
• W2077189905 created "2016-06-24" @default.
• W2077189905 creator A5005266449 @default.
• W2077189905 creator A5006096067 @default.
• W2077189905 creator A5027099010 @default.
• W2077189905 creator A5041669055 @default.
• W2077189905 creator A5077337299 @default.
• W2077189905 creator A5077373617 @default.
• W2077189905 creator A5077652405 @default.
• W2077189905 date "2013-07-11" @default.
• W2077189905 modified "2023-09-27" @default.
• W2077189905 title "Crosslinking and mass spectrometry suggest that the isolated NTD domain dimer of Moloney murine leukemia virus integrase adopts a parallel arrangement in solution" @default.
• W2077189905 cites W1977741397 @default.
• W2077189905 cites W1988330692 @default.
• W2077189905 cites W1991057723 @default.
• W2077189905 cites W1992151403 @default.
• W2077189905 cites W2001131743 @default.
• W2077189905 cites W2012534919 @default.
• W2077189905 cites W2017997170 @default.
• W2077189905 cites W2018272940 @default.
• W2077189905 cites W2020555083 @default.
• W2077189905 cites W2025344323 @default.
• W2077189905 cites W2026268551 @default.
• W2077189905 cites W2032891258 @default.
• W2077189905 cites W2035324380 @default.
• W2077189905 cites W2043638353 @default.
• W2077189905 cites W2044660041 @default.
• W2077189905 cites W2056101310 @default.
• W2077189905 cites W2060369798 @default.
• W2077189905 cites W2071812170 @default.
• W2077189905 cites W2073565105 @default.
• W2077189905 cites W2106246537 @default.
• W2077189905 cites W2113540822 @default.
• W2077189905 cites W2117072114 @default.
• W2077189905 cites W2127310354 @default.
• W2077189905 cites W2134358295 @default.
• W2077189905 cites W2139647182 @default.
• W2077189905 cites W2164479436 @default.
• W2077189905 cites W2170129382 @default.
• W2077189905 doi "https://doi.org/10.1186/1472-6807-13-14" @default.
• W2077189905 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3750625" @default.
• W2077189905 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23844665" @default.
• W2077189905 hasPublicationYear "2013" @default.
• W2077189905 type Work @default.
• W2077189905 sameAs 2077189905 @default.
• W2077189905 citedByCount "2" @default.
• W2077189905 countsByYear W20771899052015 @default.
• W2077189905 countsByYear W20771899052018 @default.
• W2077189905 crossrefType "journal-article" @default.
• W2077189905 hasAuthorship W2077189905A5005266449 @default.
• W2077189905 hasAuthorship W2077189905A5006096067 @default.
• W2077189905 hasAuthorship W2077189905A5027099010 @default.
• W2077189905 hasAuthorship W2077189905A5041669055 @default.
• W2077189905 hasAuthorship W2077189905A5077337299 @default.
• W2077189905 hasAuthorship W2077189905A5077373617 @default.
• W2077189905 hasAuthorship W2077189905A5077652405 @default.
• W2077189905 hasBestOaLocation W20771899051 @default.
• W2077189905 hasConcept C104317684 @default.
• W2077189905 hasConcept C142462285 @default.
• W2077189905 hasConcept C14430832 @default.
• W2077189905 hasConcept C159047783 @default.
• W2077189905 hasConcept C166940927 @default.
• W2077189905 hasConcept C178790620 @default.
• W2077189905 hasConcept C181199279 @default.
• W2077189905 hasConcept C185592680 @default.
• W2077189905 hasConcept C2778886173 @default.
• W2077189905 hasConcept C2779502633 @default.
• W2077189905 hasConcept C2779546866 @default.
• W2077189905 hasConcept C2909909245 @default.
• W2077189905 hasConcept C2993143319 @default.
• W2077189905 hasConcept C3013748606 @default.
• W2077189905 hasConcept C38441620 @default.
• W2077189905 hasConcept C521977710 @default.
• W2077189905 hasConcept C552990157 @default.
• W2077189905 hasConcept C55493867 @default.
• W2077189905 hasConcept C67705224 @default.
• W2077189905 hasConcept C71240020 @default.
• W2077189905 hasConcept C86803240 @default.
• W2077189905 hasConceptScore W2077189905C104317684 @default.
• W2077189905 hasConceptScore W2077189905C142462285 @default.
• W2077189905 hasConceptScore W2077189905C14430832 @default.
• W2077189905 hasConceptScore W2077189905C159047783 @default.
• W2077189905 hasConceptScore W2077189905C166940927 @default.
• W2077189905 hasConceptScore W2077189905C178790620 @default.
• W2077189905 hasConceptScore W2077189905C181199279 @default.
• W2077189905 hasConceptScore W2077189905C185592680 @default.
• W2077189905 hasConceptScore W2077189905C2778886173 @default.
• W2077189905 hasConceptScore W2077189905C2779502633 @default.
• W2077189905 hasConceptScore W2077189905C2779546866 @default.
• W2077189905 hasConceptScore W2077189905C2909909245 @default.
• W2077189905 hasConceptScore W2077189905C2993143319 @default.
• W2077189905 hasConceptScore W2077189905C3013748606 @default.
• W2077189905 hasConceptScore W2077189905C38441620 @default.
• W2077189905 hasConceptScore W2077189905C521977710 @default.
• W2077189905 hasConceptScore W2077189905C552990157 @default.
• W2077189905 hasConceptScore W2077189905C55493867 @default.
• W2077189905 hasConceptScore W2077189905C67705224 @default.
• W2077189905 hasConceptScore W2077189905C71240020 @default.
• W2077189905 hasConceptScore W2077189905C86803240 @default.
• W2077189905 hasIssue "1" @default.

• next