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- W2077237347 abstract "We analyzed the effect of ethidium bromide (EtBr) on the cleavage of closed circular pBR322 DNA molecules by six restriction enzymes which make staggered or flush cuts (EcoRI, HindIII, BglI, PstI, HincII, PvuII). EtBr concentrations and reaction temperatures were determined at which DNA molecules with single-strand breaks were the major reaction product of digestion by all the enzymes. However, the amounts of intermediates which could be isolated differed for various enzymes. The results extend previous studies, showing that sequential cleavage of the DNA strands probably is a general property of restriction endonucleases." @default.
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- W2077237347 date "1982-11-01" @default.
- W2077237347 modified "2023-10-15" @default.
- W2077237347 title "Ethidium-bromide-inhibited restriction endonucleases cleave one strand of circular DNA" @default.
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- W2077237347 doi "https://doi.org/10.1016/0378-1119(82)90093-2" @default.
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