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• W2077261712 abstract "The glutaminyl-tRNA synthetase (GlnRS) enzyme, which pairs glutamine with tRNAGln for protein synthesis, evolved by gene duplication in early eukaryotes from a nondiscriminating glutamyl-tRNA synthetase (GluRS) that aminoacylates both tRNAGln and tRNAGlu with glutamate. This ancient GluRS also separately differentiated to exclude tRNAGln as a substrate, and the resulting discriminating GluRS and GlnRS further acquired additional protein domains assisting function in cis (the GlnRS N-terminal Yqey domain) or in trans (the Arc1p protein associating with GluRS). These added domains are absent in contemporary bacterial GlnRS and GluRS. Here, using Saccharomyces cerevisiae enzymes as models, we find that the eukaryote-specific protein domains substantially influence amino acid binding, tRNA binding and aminoacylation efficiency, but they play no role in either specific nucleotide readout or discrimination against noncognate tRNA. Eukaryotic tRNAGln and tRNAGlu recognition determinants are found in equivalent positions and are mutually exclusive to a significant degree, with key nucleotides located adjacent to portions of the protein structure that differentiated during the evolution of archaeal nondiscriminating GluRS to GlnRS. These findings provide important corroboration for the evolutionary model and suggest that the added eukaryotic domains arose in response to distinctive selective pressures associated with the greater complexity of the eukaryotic translational apparatus. We also find that the affinity of GluRS for glutamate is significantly increased when Arc1p is not associated with the enzyme. This is consistent with the lower concentration of intracellular glutamate and the dissociation of the Arc1p:GluRS complex upon the diauxic shift to respiratory conditions." @default.
• W2077261712 created "2016-06-24" @default.
• W2077261712 creator A5023336794 @default.
• W2077261712 creator A5056156015 @default.
• W2077261712 date "2014-10-01" @default.
• W2077261712 modified "2023-10-14" @default.
• W2077261712 title "Coevolution of Specificity Determinants in Eukaryotic Glutamyl- and Glutaminyl-tRNA Synthetases" @default.
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• W2077261712 cites W174069803 @default.
• W2077261712 cites W1774953805 @default.
• W2077261712 cites W1966174219 @default.
• W2077261712 cites W1966916474 @default.
• W2077261712 cites W1968202297 @default.
• W2077261712 cites W1969170660 @default.
• W2077261712 cites W1973290333 @default.
• W2077261712 cites W1973772482 @default.
• W2077261712 cites W1991324280 @default.
• W2077261712 cites W1997128348 @default.
• W2077261712 cites W1997648346 @default.
• W2077261712 cites W2001373414 @default.
• W2077261712 cites W2003646873 @default.
• W2077261712 cites W2005553519 @default.
• W2077261712 cites W2005747480 @default.
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• W2077261712 cites W2036130241 @default.
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• W2077261712 cites W2071213340 @default.
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• W2077261712 cites W2136201971 @default.
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• W2077261712 cites W2147302373 @default.
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• W2077261712 doi "https://doi.org/10.1016/j.jmb.2014.08.006" @default.
• W2077261712 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25149203" @default.
• W2077261712 hasPublicationYear "2014" @default.
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