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• W2077277848 abstract "The presence and importance of microenvironments in the subsurface at contaminated sites were suggested by previous geochemical studies. However, no direct quantitative characterization of the geochemical microenvironments had been reported. We quantitatively characterized microscale geochemical gradients (dissolved oxygen (DO), H2, pH, and redox potential) in Hanford 300A subsurface sediment biofilms. Our results revealed significant differences in geochemical parameters across the sediment biofilm/water interface in the presence and absence of U(VI) under oxic and anoxic conditions. While the pH was relatively constant within the sediment biofilm, the redox potential and the DO and H2 concentrations were heterogeneous at the microscale (<500–1000 μm). We found microenvironments with high DO levels (DO hotspots) when the sediment biofilm was exposed to U(VI). On the other hand, we found hotspots (high concentrations) of H2 under anoxic conditions both in the presence and in the absence of U(VI). The presence of anoxic microenvironments inside the sediment biofilms suggests that U(VI) reduction proceeds under bulk oxic conditions. To test this, we operated our biofilm reactor under air-saturated conditions in the presence of U(VI) and characterized U speciation in the sediment biofilm. U LIII-edge X-ray absorption spectroscopy (XANES and EXAFS) showed that 80–85% of the U was in the U(IV) valence state." @default.
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• W2077277848 date "2012-01-01" @default.
• W2077277848 modified "2023-10-14" @default.
• W2077277848 title "Microscale geochemical gradients in Hanford 300 Area sediment biofilms and influence of uranium" @default.
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• W2077277848 doi "https://doi.org/10.1016/j.watres.2011.10.054" @default.
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