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• W2077304300 abstract "Microtubules (MTs) are essential components of the cytoskeleton that play critical roles in neurodevelopment and adaptive central nervous system functioning. MTs are essential to growth cone advance and ultrastructural events integral to synaptic plasticity; these functions figure significantly into current pathophysiologic conceptualizations of schizophrenia. To date, no study has directly investigated MT dynamics in humans with schizophrenia. We therefore compared the stability of MTs in olfactory neuroepithelial (OE) cells between schizophrenia cases and matched nonpsychiatric comparison subjects. For this purpose, we applied nocodazole (Nz) to cultured OE cells obtained from tissue biopsies from seven living schizophrenia patients and seven matched comparison subjects; all schizophrenia cases were on antipsychotic medications. Nz allows MT depolymerization to be followed but prevents repolymerization, so that in living cells treated for varying time intervals, the MTs that are stable for a given treatment interval remain. Our readout of MT stability was the time at which fewer than 10 MTs per cell could be distinguished by anti-β-tubulin immunofluorescence. The percentage of cells with ≥10 intact MTs at specified intervals following Nz treatment was estimated by systematic uniform random sampling with Visiopharm software. These analyses showed that the mean percentages of OE cells with intact MTs were significantly greater for schizophrenia cases than for the matched comparison subjects at 10, 15, and 30min following Nz treatment indicating increased MT stability in OE cells from schizophrenia patients (p=0.0007 at 10min; p=0.0008 at 15min; p=0.036 at 30min). In conclusion, we have demonstrated increased MT stability in nearly all cultures of OE cells from individuals with schizophrenia, who received several antipsychotic treatments, versus comparison subjects matched for age and sex. While we cannot rule out a possible confounding effect of antipsychotic medications, these findings may reflect analogous neurobiological events in at least a subset of immature neurons or other cell types during gestation, or newly generated cells destined for the olfactory bulb or hippocampus, suggesting a mechanism that underlies findings of postmortem and neuroimaging investigations of schizophrenia. Future studies aimed at replicating these findings, including samples of medication-naïve subjects with schizophrenia, and reconciling the results with other studies, will be necessary. Although the observed abnormalities may suggest one of a number of putative pathophysiologic anomalies in schizophrenia, this work may ultimately have implications for an improved understanding of pathogenic processes related to this disorder." @default.
• W2077304300 created "2016-06-24" @default.
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• W2077304300 date "2014-01-01" @default.
• W2077304300 modified "2023-09-28" @default.
• W2077304300 title "Increased stability of microtubules in cultured olfactory neuroepithelial cells from individuals with schizophrenia" @default.
• W2077304300 cites W1965118964 @default.
• W2077304300 cites W1966975951 @default.
• W2077304300 cites W1967888910 @default.
• W2077304300 cites W1969141861 @default.
• W2077304300 cites W1969557614 @default.
• W2077304300 cites W1970479888 @default.
• W2077304300 cites W1971696611 @default.
• W2077304300 cites W1973333398 @default.
• W2077304300 cites W1974262619 @default.
• W2077304300 cites W1975586358 @default.
• W2077304300 cites W1986171966 @default.
• W2077304300 cites W1988486070 @default.
• W2077304300 cites W1990591515 @default.
• W2077304300 cites W1992073044 @default.
• W2077304300 cites W1998420327 @default.
• W2077304300 cites W2002192892 @default.
• W2077304300 cites W2002305171 @default.
• W2077304300 cites W2005402647 @default.
• W2077304300 cites W2009233191 @default.
• W2077304300 cites W2013583898 @default.
• W2077304300 cites W2019582024 @default.
• W2077304300 cites W2021958153 @default.
• W2077304300 cites W2024035512 @default.
• W2077304300 cites W2029437594 @default.
• W2077304300 cites W2030446659 @default.
• W2077304300 cites W2030878563 @default.
• W2077304300 cites W2032100189 @default.
• W2077304300 cites W2032591020 @default.
• W2077304300 cites W2049221816 @default.
• W2077304300 cites W2050511573 @default.
• W2077304300 cites W2053533857 @default.
• W2077304300 cites W2054564838 @default.
• W2077304300 cites W2057565196 @default.
• W2077304300 cites W2061267887 @default.
• W2077304300 cites W2066578425 @default.
• W2077304300 cites W2070140643 @default.
• W2077304300 cites W2073440202 @default.
• W2077304300 cites W2085401189 @default.
• W2077304300 cites W2089428712 @default.
• W2077304300 cites W2091485668 @default.
• W2077304300 cites W2095498339 @default.
• W2077304300 cites W2095546080 @default.
• W2077304300 cites W2098391962 @default.
• W2077304300 cites W2099636437 @default.
• W2077304300 cites W2103592460 @default.
• W2077304300 cites W2109807917 @default.
• W2077304300 cites W2117633779 @default.
• W2077304300 cites W2121994499 @default.
• W2077304300 cites W2122694225 @default.
• W2077304300 cites W2122715226 @default.
• W2077304300 cites W2125788186 @default.
• W2077304300 cites W2126688513 @default.
• W2077304300 cites W2130070111 @default.
• W2077304300 cites W2130987622 @default.
• W2077304300 cites W2132015070 @default.
• W2077304300 cites W2135114865 @default.
• W2077304300 cites W2135744973 @default.
• W2077304300 cites W2141734192 @default.
• W2077304300 cites W2142697421 @default.
• W2077304300 cites W2144874509 @default.
• W2077304300 cites W2146264275 @default.
• W2077304300 cites W2149246707 @default.
• W2077304300 cites W2154968967 @default.
• W2077304300 cites W2155768517 @default.
• W2077304300 cites W2156305088 @default.
• W2077304300 cites W2159400463 @default.
• W2077304300 cites W2159829507 @default.
• W2077304300 cites W2162105640 @default.
• W2077304300 cites W2162916231 @default.
• W2077304300 cites W2165310329 @default.
• W2077304300 cites W2169726711 @default.
• W2077304300 cites W2325116203 @default.
• W2077304300 doi "https://doi.org/10.1016/j.pnpbp.2013.10.015" @default.
• W2077304300 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3999307" @default.
• W2077304300 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24513021" @default.
• W2077304300 hasPublicationYear "2014" @default.
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