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• W2077308230 endingPage "7501" @default.
• W2077308230 startingPage "7491" @default.
• W2077308230 abstract "The nucleotide sequence of a 2.5 kb segment of the pKM1Ol (R46) genome has been determined. The 1.3 kb from a BamHl site at 153 to base 1440 differs by only 2 bases from a part of the published sequence (1) of the aadB (gentamicin resistance) gene region including the coding region for the N-terminal 70 amino acids of the predicted aadB product. The same sequence has been found (1) 5′-to the dhfrll gene of R388 and to the aadA gene of Tn27 (R538-1). Three open reading frames are located in this region, two on the same strand as the resistance genes and one on the complementary strand. The latter predicts a polypeptide of 337 amino acids, whose N-terminal segment is 40% homologous to the predicted product of an open reading frame of 179 amino acids located next to the dhfrl gene of Tn7 (2). The oxa2 (oxacillin resistance) gene predicts a long polypeptide commencing with (the N-terminal) 70 amino acids of the aadB product A similar arrangement is found in the aadA gene of R538-1 (3). The N-terminal segment of an aadA gene is located 3′- to axa2, separated by 36 bases. Sequences surrounding the BamHl site are identical to sequences 5′- to the tnpM gene of Tn27 (4, 5) and homology ceases where homology between Tn27 and Tn501 commences (4). The possibility that this antibiotic resistance segment is a discrete mobile DNA element is discussed." @default.
• W2077308230 created "2016-06-24" @default.
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• W2077308230 date "1987-01-01" @default.
• W2077308230 modified "2023-10-06" @default.
• W2077308230 title "The region of the incN plasmid R46 coding for resistance to β-lactam antibiotics, streptomycin/spectinomycin and sulphonamides is closely related to antibiotic resistance segments found In IncW plasm ids and in Tn21-like transposons" @default.
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• W2077308230 doi "https://doi.org/10.1093/nar/15.18.7491" @default.
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