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- W2077308968 abstract "Transport of l-glutamic acid into the fission yeast Schizosaccharomyces pombe grown to the early stationary phase and preincubated for 60 min with 1% d-glucose is practically unidirectiional and is mediated by a single uphill transport system with a KT of 170 μM and Jmax of 4.8 nmol min−1 (mg dry wt.)−1. The system proved to be rather non-specific since all the amino acids transported into the cells acted as potent competitive inhibitors. It has a pH optimum at 3.0–4.0, the accumulation ratio of l-glutamic acid is highest at a suspension density of 0.6–1.0 mg dry wt. per ml and decreases with increasing l-glutamic acid concentrations in the external medium. The system present in the cells after preincubation with d-glucose is unstable and its activity decays after washing the cells with water or after stopping the cytosolic proteinsynthesis with cycloheximide, with a half-time of 24 min in a reaction significantly retarded by phenylmethylsulfonyl fluoride, a serine proteinase inhibitor. The synthesis of the transport protein appears to be repressible by ammonium ions." @default.
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- W2077308968 title "Transport of l-glutamic acid in the fission yeast Schizosaccharomyces pombe" @default.
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- W2077308968 doi "https://doi.org/10.1016/0005-2736(92)90088-4" @default.
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