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- W2077309045 abstract "Radioactively labeled N-[1- 14 C]-octanoyll-sphinganine and D-erythro-[3- 3 H]-sphinganine were administered in parallel experiments to neuroblastoma cells B 104. A time dependent formation of ceramide with a double bond in its sphingoid backbone was observed in both cases. In the presence of fumonisin B 1 (25 μM), a strong inhibitor of sphinganine N-acyltransferase, desaturated ceramide was formed only when cells were fed with N-[1- 14 C]-octanoyl-sphinganine but not with [3- 3 H]-sphinganine. Thus, the introduction of the double bond occurs only at the level of dihydroceramide, after N-acylation of sphinganine. It is now obvious that sphingosine is not a biosynthetic intermediate but exclusively a catabolic product of cellular sphingolipids." @default.
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- W2077309045 date "1992-11-01" @default.
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- W2077309045 title "Biosynthesis of sphingolipids: Dihydroceramide and not sphinganine is desaturated by cultured cells" @default.
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- W2077309045 doi "https://doi.org/10.1016/0006-291x(92)91518-u" @default.
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