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• W2077338510 abstract "Glucose degradation products (GDP) retard remesothelialization independently of D-glucose concentration.BackgroundGlucose degradation products (GDP) present in heat-sterilized dialysis fluids are thought to contribute to cellular dysfunction and membrane damage during peritoneal dialysis. To examine the effects of specific GDP on the remesothelialization process, the impact of conventional and low GDP peritoneal dialysis solutions, d-glucose, and individual GDP in a scratch-wounding model was assessed.MethodsScratch (0.5 to 0.6 mm)-wounded human peritoneal mesothelial cells (HPMC) were treated, at pH 7.4, with either (1) control medium (M199), (2) laboratory-prepared heat or filter-sterilized solutions, (3) 10% to 80% vol/vol solution of Gambrosol® or Gambrosol-trio® (1.5% and 4.0% glucose), (4) d-glucose (5 to 80 mmol/L), or (5) individual or combined GDP [acetaldehyde, formaldehyde, glyoxal, methylglyoxal, 3-deoxyglucosone (3-DG), 5-hydroxy methylfufural (5-HMF), or 3,4-di-deoxyglucosone-3-ene (3,4-DGE)]. Wound closure was recorded by time-lapse photomicroscopy.ResultsIn untreated HPMC, the rate of wound closure was linear and the process was complete by 18.4 ± 3.6 hours (N = 16). In wounded HPMC exposed to dilutions of heat-sterilized but not filtered laboratory solutions (1.5% or 4.0% glucose, pH 7.4), remesothelialization was significantly retarded (P = 0.04 and P = 0.009 vs. M199, respectively). In Gambrosol®, remesothelialization was significantly retarded in both 1.5% and 4.0% solutions. In contrast in Gambrosol-trio®–treated HPMC, this rate was not significantly reduced in either 1.5% or 4.0% glucose peritoneal dialysis fluids. Remesothelialization was dose-dependently retarded in HPMC exposed to 3,4-DGE (>10 μmol/L), formaldehyde (>5 μmol/L) but not by exposure to the other GDP tested even at 5 times the concentration present in low glucose solutions. The rate of remesothelialization was not significantly altered by exposure to d-glucose concentrations up to 80 mmol/L.ConclusionThese data identify that the formaldehyde and 3,4-DGE present in heat-sterilized peritoneal dialysis solutions are important in reducing mesothelial cell regeneration. Specifically targeting their removal may have major benefits in preserving the mesothelium during long-term peritoneal dialysis. Glucose degradation products (GDP) retard remesothelialization independently of D-glucose concentration. Glucose degradation products (GDP) present in heat-sterilized dialysis fluids are thought to contribute to cellular dysfunction and membrane damage during peritoneal dialysis. To examine the effects of specific GDP on the remesothelialization process, the impact of conventional and low GDP peritoneal dialysis solutions, d-glucose, and individual GDP in a scratch-wounding model was assessed. Scratch (0.5 to 0.6 mm)-wounded human peritoneal mesothelial cells (HPMC) were treated, at pH 7.4, with either (1) control medium (M199), (2) laboratory-prepared heat or filter-sterilized solutions, (3) 10% to 80% vol/vol solution of Gambrosol® or Gambrosol-trio® (1.5% and 4.0% glucose), (4) d-glucose (5 to 80 mmol/L), or (5) individual or combined GDP [acetaldehyde, formaldehyde, glyoxal, methylglyoxal, 3-deoxyglucosone (3-DG), 5-hydroxy methylfufural (5-HMF), or 3,4-di-deoxyglucosone-3-ene (3,4-DGE)]. Wound closure was recorded by time-lapse photomicroscopy. In untreated HPMC, the rate of wound closure was linear and the process was complete by 18.4 ± 3.6 hours (N = 16). In wounded HPMC exposed to dilutions of heat-sterilized but not filtered laboratory solutions (1.5% or 4.0% glucose, pH 7.4), remesothelialization was significantly retarded (P = 0.04 and P = 0.009 vs. M199, respectively). In Gambrosol®, remesothelialization was significantly retarded in both 1.5% and 4.0% solutions. In contrast in Gambrosol-trio®–treated HPMC, this rate was not significantly reduced in either 1.5% or 4.0% glucose peritoneal dialysis fluids. Remesothelialization was dose-dependently retarded in HPMC exposed to 3,4-DGE (>10 μmol/L), formaldehyde (>5 μmol/L) but not by exposure to the other GDP tested even at 5 times the concentration present in low glucose solutions. The rate of remesothelialization was not significantly altered by exposure to d-glucose concentrations up to 80 mmol/L. These data identify that the formaldehyde and 3,4-DGE present in heat-sterilized peritoneal dialysis solutions are important in reducing mesothelial cell regeneration. Specifically targeting their removal may have major benefits in preserving the mesothelium during long-term peritoneal dialysis." @default.
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• W2077338510 date "2003-11-01" @default.
• W2077338510 modified "2023-10-10" @default.
• W2077338510 title "Glucose degradation products (GDP) retard remesothelialization independently of d-glucose concentration" @default.
• W2077338510 cites W1488288160 @default.
• W2077338510 cites W1894989396 @default.
• W2077338510 cites W1936888460 @default.
• W2077338510 cites W1966851176 @default.
• W2077338510 cites W1966999635 @default.
• W2077338510 cites W1971008553 @default.
• W2077338510 cites W1976166903 @default.
• W2077338510 cites W1982379205 @default.
• W2077338510 cites W1985568439 @default.
• W2077338510 cites W1991272197 @default.
• W2077338510 cites W1991580413 @default.
• W2077338510 cites W1992715660 @default.
• W2077338510 cites W2009818543 @default.
• W2077338510 cites W2012276221 @default.
• W2077338510 cites W2023543604 @default.
• W2077338510 cites W2027246301 @default.
• W2077338510 cites W2030234335 @default.
• W2077338510 cites W2041101287 @default.
• W2077338510 cites W2049599823 @default.
• W2077338510 cites W2052640088 @default.
• W2077338510 cites W2060256756 @default.
• W2077338510 cites W2060368510 @default.
• W2077338510 cites W2060494754 @default.
• W2077338510 cites W2066579306 @default.
• W2077338510 cites W2077865487 @default.
• W2077338510 cites W2080442169 @default.
• W2077338510 cites W2084404396 @default.
• W2077338510 cites W2084649332 @default.
• W2077338510 cites W2085423967 @default.
• W2077338510 cites W2088726500 @default.
• W2077338510 cites W2091384424 @default.
• W2077338510 cites W2097357683 @default.
• W2077338510 cites W2108870136 @default.
• W2077338510 cites W2118254829 @default.
• W2077338510 cites W2123397465 @default.
• W2077338510 cites W2123600421 @default.
• W2077338510 cites W2127376808 @default.
• W2077338510 cites W2132914950 @default.
• W2077338510 cites W2133022777 @default.
• W2077338510 cites W2136081838 @default.
• W2077338510 cites W2136465314 @default.
• W2077338510 cites W2157196487 @default.
• W2077338510 cites W2160816281 @default.
• W2077338510 cites W2168305895 @default.
• W2077338510 cites W2168904251 @default.
• W2077338510 cites W2169085200 @default.
• W2077338510 cites W2181615208 @default.
• W2077338510 cites W2190630543 @default.
• W2077338510 cites W2192080100 @default.
• W2077338510 cites W2337479359 @default.
• W2077338510 cites W2341141705 @default.
• W2077338510 cites W2346692818 @default.
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• W2077338510 doi "https://doi.org/10.1046/j.1523-1755.2003.00265.x" @default.
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