Matches in SemOpenAlex for { <https://semopenalex.org/work/W2077351076> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2077351076 endingPage "85" @default.
- W2077351076 startingPage "80" @default.
- W2077351076 abstract "Current procedures for solubilization of membrane proteins involve the use of detergents. A procedure using high hydrostatic pressures without detergent has been applied in this study to subfractionate membrane proteins and their endogenously associated lipids. Rat brain membrane preparations were suspended in hypotonic buffer containing the membrane fluidizer benzyl alcohol in a sealed pressure cell and subjected to hydrostatic pressures of up to 1500 atmospheres (approx 22,000 psi) in a French press. Under these conditions, specific membrane proteins including protein kinase C, phospholipase A2, calmodulin-binding proteins, G-proteins, and microtubule-associated proteins all coextracted and were associated to lipid particles, suggesting inherent physical contact. Two populations of membrane-associated protein kinase C were identified according to molecular weight estimations. The first coeluted with the lipid particles composed predominantly of phospholipids, while the second contained much less lipid and was similar to the soluble monomer, i.e., cytosolic protein kinase C. This procedure provides an important technique for selective subfractionation of membrane proteins in their native lipid environment which could be used for structure-function studies." @default.
- W2077351076 created "2016-06-24" @default.
- W2077351076 creator A5010014902 @default.
- W2077351076 creator A5017829582 @default.
- W2077351076 creator A5035637931 @default.
- W2077351076 creator A5074933659 @default.
- W2077351076 date "1990-11-01" @default.
- W2077351076 modified "2023-09-24" @default.
- W2077351076 title "Application of high-pressure to subfractionate membrane protein-lipid complexes: A case study of protein kinase C" @default.
- W2077351076 cites W1499460529 @default.
- W2077351076 cites W1586296071 @default.
- W2077351076 cites W1634621704 @default.
- W2077351076 cites W1797391587 @default.
- W2077351076 cites W1949772429 @default.
- W2077351076 cites W1976459328 @default.
- W2077351076 cites W1985533454 @default.
- W2077351076 cites W1989675515 @default.
- W2077351076 cites W1990849016 @default.
- W2077351076 cites W2002984440 @default.
- W2077351076 cites W2020220074 @default.
- W2077351076 cites W2025295746 @default.
- W2077351076 cites W2061216302 @default.
- W2077351076 cites W2072081577 @default.
- W2077351076 cites W2088533660 @default.
- W2077351076 cites W2111726538 @default.
- W2077351076 cites W2134634622 @default.
- W2077351076 cites W2168526937 @default.
- W2077351076 cites W2201707750 @default.
- W2077351076 cites W4293247451 @default.
- W2077351076 doi "https://doi.org/10.1016/0003-2697(90)90391-l" @default.
- W2077351076 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2077943" @default.
- W2077351076 hasPublicationYear "1990" @default.
- W2077351076 type Work @default.
- W2077351076 sameAs 2077351076 @default.
- W2077351076 citedByCount "24" @default.
- W2077351076 countsByYear W20773510762014 @default.
- W2077351076 countsByYear W20773510762016 @default.
- W2077351076 countsByYear W20773510762020 @default.
- W2077351076 crossrefType "journal-article" @default.
- W2077351076 hasAuthorship W2077351076A5010014902 @default.
- W2077351076 hasAuthorship W2077351076A5017829582 @default.
- W2077351076 hasAuthorship W2077351076A5035637931 @default.
- W2077351076 hasAuthorship W2077351076A5074933659 @default.
- W2077351076 hasConcept C107565407 @default.
- W2077351076 hasConcept C144647389 @default.
- W2077351076 hasConcept C161200384 @default.
- W2077351076 hasConcept C166311969 @default.
- W2077351076 hasConcept C170493617 @default.
- W2077351076 hasConcept C181199279 @default.
- W2077351076 hasConcept C184235292 @default.
- W2077351076 hasConcept C185592680 @default.
- W2077351076 hasConcept C195794163 @default.
- W2077351076 hasConcept C24530287 @default.
- W2077351076 hasConcept C41625074 @default.
- W2077351076 hasConcept C55493867 @default.
- W2077351076 hasConcept C560191 @default.
- W2077351076 hasConcept C97029542 @default.
- W2077351076 hasConcept C98539663 @default.
- W2077351076 hasConceptScore W2077351076C107565407 @default.
- W2077351076 hasConceptScore W2077351076C144647389 @default.
- W2077351076 hasConceptScore W2077351076C161200384 @default.
- W2077351076 hasConceptScore W2077351076C166311969 @default.
- W2077351076 hasConceptScore W2077351076C170493617 @default.
- W2077351076 hasConceptScore W2077351076C181199279 @default.
- W2077351076 hasConceptScore W2077351076C184235292 @default.
- W2077351076 hasConceptScore W2077351076C185592680 @default.
- W2077351076 hasConceptScore W2077351076C195794163 @default.
- W2077351076 hasConceptScore W2077351076C24530287 @default.
- W2077351076 hasConceptScore W2077351076C41625074 @default.
- W2077351076 hasConceptScore W2077351076C55493867 @default.
- W2077351076 hasConceptScore W2077351076C560191 @default.
- W2077351076 hasConceptScore W2077351076C97029542 @default.
- W2077351076 hasConceptScore W2077351076C98539663 @default.
- W2077351076 hasIssue "1" @default.
- W2077351076 hasLocation W20773510761 @default.
- W2077351076 hasLocation W20773510762 @default.
- W2077351076 hasOpenAccess W2077351076 @default.
- W2077351076 hasPrimaryLocation W20773510761 @default.
- W2077351076 hasRelatedWork W1481062332 @default.
- W2077351076 hasRelatedWork W1976484542 @default.
- W2077351076 hasRelatedWork W2011366293 @default.
- W2077351076 hasRelatedWork W2023163561 @default.
- W2077351076 hasRelatedWork W2107584639 @default.
- W2077351076 hasRelatedWork W2128882835 @default.
- W2077351076 hasRelatedWork W2156144788 @default.
- W2077351076 hasRelatedWork W2487646191 @default.
- W2077351076 hasRelatedWork W2491853606 @default.
- W2077351076 hasRelatedWork W83500288 @default.
- W2077351076 hasVolume "191" @default.
- W2077351076 isParatext "false" @default.
- W2077351076 isRetracted "false" @default.
- W2077351076 magId "2077351076" @default.
- W2077351076 workType "article" @default.