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• W2077361215 abstract "The effects of native thrombospondin (TSP), an 18 kd recombinant protein comprising residues 1–174 of TSP (TSP1–174) with heparin-binding domain and a fusion protein comprising residues 559–669 of TSP (TSP559–669) on murine hematopoiesis, were studied by using different in vitro culture systems. TSP by itself did not show an inhibitory effect on colony-forming unit-megakaryocyte (CFU-MK) growth in a serum-free agar system and on the growth of colony-forming unit-granulocyte and macrophage (CFU-GM) in a plasma clot system. It was, however, found that in the plasma clot culture system when using aplastic anemia serum as the source of thrombopoietin or C-Mpl ligand (TPO), TSP and TSP1–174, but not TSP559–669, were able to inhibit the growth of CFU-MK from unfractionated and lineage negative (Lin-) bone marrow cells in a dose-dependent manner. A statistically significant suppression was seen at 1 μg/ml of TSP and 5 μg/ml of TSP1–174. This inhibitory effect of TSP was further found in both the serum-free agar system and the plasma clot system without aplastic anemia serum, where megakaryocyte colony growth was stimulated by recombinant TPO, basic fibroblast growth factor (bFGF), or interleukin-3 (IL-3). In a methylcellulose system, where a combination of stem cell factor (SCF), IL-3, and granulocyte/macrophage colony-stimulating factor (GM-CSF) were used, TSP inhibited the growth of colony-forming unit-granulocyte-erythroblast, megakaryocyte, and macrophage (CFU-GEMM) but not CFU-GM and burst-forming unit-erythroblast (BFU-E). Interestingly, this inhibitory effect of TSP on megakaryocyte colony growth could be counteracted by Fraxiparin, a low-molecular-weight heparin. These results demonstrate that TSP is a negative modulator of megakaryocytopoiesis and suggest that its inhibitory effect is at least partially mediated by N-terminal heparin-binding domain." @default.
• W2077361215 created "2016-06-24" @default.
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• W2077361215 date "1997-02-01" @default.
• W2077361215 modified "2023-10-18" @default.
• W2077361215 title "Thrombospondin, a negative modulator of megakaryocytopoiesis" @default.
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• W2077361215 doi "https://doi.org/10.1016/s0022-2143(97)90144-x" @default.
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