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- W2077384752 abstract "Two DNA polymerase activities, A and B, have been separated from rat brain. The activities of both fractions depended on the presence of all four deoxyribonucleoside triphosphates, Mg2+ and primer DNA. The optimal concentration of Mg2+ was 3–5 mM and optimal pH was around 7.5 for both Enzymes A and B. However, some properties of these two enzymes differed considerably. Enzyme A showed a 2–3 times preference for heat-denatured DNA and Enzyme B was 6–30 times more active with native DNA than with heat-denatured DNA as template. The optimal concentration of Mn2+ was 0.05 mM for Enzyme A and 0.3 mM for Enzyme B. Enzyme A was more sensitive to dithiothreitol. The reaction levelled off after 40 min incubation with Enzyme A but incorporation was linear for at least 2 h with Enzyme B. Polyamines activated Enzyme B more than 50 % but had slight or no effect on Enzyme A." @default.
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- W2077384752 date "1971-08-01" @default.
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- W2077384752 title "Separation and properties of DNA polymerases from developing RAT brain" @default.
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- W2077384752 doi "https://doi.org/10.1016/0005-2787(71)90070-0" @default.
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