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- W2077387334 abstract "In this contribution we present a novel LED-photodiode based infrared absorbance sensor in the wavelength range of 3.0 - 3.7 μm for cell analysis. Instead of using time consuming and expensive labelling and staining techniques to distinguish healthy from malignant cell types, this IR sensor system can perform faster, cheaper and without the need of additional chemicals. Depending on the used narrow bandpass filters, absorbance due to specific molecular vibration can be measured, such as the functional absorbance peaks at 3.38 μm (CH<sub>3</sub>-antisymmetric stretch), 3.42 μm (CH<sub>2</sub>- antisymmetric stretch), 3.48 μm (CH<sub>3</sub>-symmetric stretch) and 3.51 μm (CH<sub>2</sub>-symmetric stretch). For normalization and baseline correction the absorbance at wavelengths 3.33 and 3.57 μm are used. By recording the IR absorbance spectra of healthy and malignant epithelial kidney cell lines with an IR spectroscope, we found significant differences in the absorbance ratio 3.51 μm / 3.42 μm (CH<sub>2</sub>-symmetric/antisymmetric stretch). This result has led us to a sensor concept where only four wavelengths are being measured. In the 3.0 - 3.7 μm wavelength region a low cost LED-photodiode system can be used instead of a spectroscope. Yeast cells, which also contain the CH<sub>2</sub> symmetric and antisymmetric stretch bands, are used to validate this sensor system and to make a first comparison of the system to spectroscopic recordings. Sensor experiments on dried spots of baker's yeast on calcium-fluoride slides yielded a comparable CH<sub>2</sub> stretch ratio with the IR spectroscope measurement. This confirms the usability of the sensor to measure the CH<sub>2</sub> stretch ratio and its potential for fast, label-free and low cost screening of cell samples." @default.
- W2077387334 created "2016-06-24" @default.
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- W2077387334 date "2009-05-20" @default.
- W2077387334 modified "2023-09-23" @default.
- W2077387334 title "3.0-3.7μm infrared sensor system for cell analysis" @default.
- W2077387334 doi "https://doi.org/10.1117/12.822058" @default.
- W2077387334 hasPublicationYear "2009" @default.
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