FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2077400416> ?p ?o ?g. }

• W2077400416 endingPage "72" @default.
• W2077400416 startingPage "66" @default.
• W2077400416 abstract "Myelin proteolipid protein (PLP) is a major integral membrane protein of central nervous system myelin and is considered to play a significant role in myelination. PLP has a four-transmembrane structure, judging from the hydropathy profile. In addition, it has InsP 6 binding activity. Here, we have succeeded in producing PLP in large quantities of 3.9 pg/cell (6 mg/L) by using a baculovirus expression system and developing an efficient purification method, maintaining InsP6 binding activity. The recombinant PLP (rPLP) was purified by ion-exchange and immunoaffinity chromatography in a non-organic solvent. The final yield of purified rPLP was 36%. The K d and B max values for the InsP 6 -PLP binding were 55 nM and 33 pmol/μg protein, respectively. The K d value of purified rPLP is equal to that of mouse brain PLP. These results indicate that purified rPLP keeps its native conformation and binds InsP 6 in an almost one-to-one ratio." @default.
• W2077400416 created "2016-06-24" @default.
• W2077400416 creator A5035555934 @default.
• W2077400416 creator A5037700663 @default.
• W2077400416 creator A5039319524 @default.
• W2077400416 creator A5046537981 @default.
• W2077400416 creator A5081662716 @default.
• W2077400416 creator A5082822809 @default.
• W2077400416 date "1998-08-01" @default.
• W2077400416 modified "2023-10-03" @default.
• W2077400416 title "Overproduction and Immuno-affinity Purification of Myelin Proteolipid Protein (PLP), an Inositol Hexakisphosphate-Binding Protein, in a Baculovirus Expression System" @default.
• W2077400416 cites W1535548654 @default.
• W2077400416 cites W1598081743 @default.
• W2077400416 cites W1967440658 @default.
• W2077400416 cites W1972262740 @default.
• W2077400416 cites W1976329033 @default.
• W2077400416 cites W1976753808 @default.
• W2077400416 cites W1978167983 @default.
• W2077400416 cites W1978274366 @default.
• W2077400416 cites W1989947994 @default.
• W2077400416 cites W1997093700 @default.
• W2077400416 cites W1998881600 @default.
• W2077400416 cites W2000597403 @default.
• W2077400416 cites W2000819264 @default.
• W2077400416 cites W2001207936 @default.
• W2077400416 cites W2014084841 @default.
• W2077400416 cites W2018011686 @default.
• W2077400416 cites W2025610870 @default.
• W2077400416 cites W2026478305 @default.
• W2077400416 cites W2029531386 @default.
• W2077400416 cites W2044274477 @default.
• W2077400416 cites W2060802834 @default.
• W2077400416 cites W2062101337 @default.
• W2077400416 cites W2066337898 @default.
• W2077400416 cites W2070193133 @default.
• W2077400416 cites W2092562744 @default.
• W2077400416 cites W2112064552 @default.
• W2077400416 cites W2119216843 @default.
• W2077400416 cites W2142850453 @default.
• W2077400416 cites W2157964279 @default.
• W2077400416 cites W2172235298 @default.
• W2077400416 cites W2187930412 @default.
• W2077400416 cites W98200847 @default.
• W2077400416 doi "https://doi.org/10.1006/bbrc.1998.8926" @default.
• W2077400416 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9705833" @default.
• W2077400416 hasPublicationYear "1998" @default.
• W2077400416 type Work @default.
• W2077400416 sameAs 2077400416 @default.
• W2077400416 citedByCount "5" @default.
• W2077400416 crossrefType "journal-article" @default.
• W2077400416 hasAuthorship W2077400416A5035555934 @default.
• W2077400416 hasAuthorship W2077400416A5037700663 @default.
• W2077400416 hasAuthorship W2077400416A5039319524 @default.
• W2077400416 hasAuthorship W2077400416A5046537981 @default.
• W2077400416 hasAuthorship W2077400416A5081662716 @default.
• W2077400416 hasAuthorship W2077400416A5082822809 @default.
• W2077400416 hasConcept C104317684 @default.
• W2077400416 hasConcept C144647389 @default.
• W2077400416 hasConcept C161200384 @default.
• W2077400416 hasConcept C169760540 @default.
• W2077400416 hasConcept C170493617 @default.
• W2077400416 hasConcept C179247698 @default.
• W2077400416 hasConcept C181199279 @default.
• W2077400416 hasConcept C185592680 @default.
• W2077400416 hasConcept C24530287 @default.
• W2077400416 hasConcept C2777426553 @default.
• W2077400416 hasConcept C2777427919 @default.
• W2077400416 hasConcept C2778609137 @default.
• W2077400416 hasConcept C2780876595 @default.
• W2077400416 hasConcept C40767141 @default.
• W2077400416 hasConcept C41625074 @default.
• W2077400416 hasConcept C43617362 @default.
• W2077400416 hasConcept C529278444 @default.
• W2077400416 hasConcept C553089730 @default.
• W2077400416 hasConcept C55493867 @default.
• W2077400416 hasConcept C7821365 @default.
• W2077400416 hasConcept C86803240 @default.
• W2077400416 hasConceptScore W2077400416C104317684 @default.
• W2077400416 hasConceptScore W2077400416C144647389 @default.
• W2077400416 hasConceptScore W2077400416C161200384 @default.
• W2077400416 hasConceptScore W2077400416C169760540 @default.
• W2077400416 hasConceptScore W2077400416C170493617 @default.
• W2077400416 hasConceptScore W2077400416C179247698 @default.
• W2077400416 hasConceptScore W2077400416C181199279 @default.
• W2077400416 hasConceptScore W2077400416C185592680 @default.
• W2077400416 hasConceptScore W2077400416C24530287 @default.
• W2077400416 hasConceptScore W2077400416C2777426553 @default.
• W2077400416 hasConceptScore W2077400416C2777427919 @default.
• W2077400416 hasConceptScore W2077400416C2778609137 @default.
• W2077400416 hasConceptScore W2077400416C2780876595 @default.
• W2077400416 hasConceptScore W2077400416C40767141 @default.
• W2077400416 hasConceptScore W2077400416C41625074 @default.
• W2077400416 hasConceptScore W2077400416C43617362 @default.
• W2077400416 hasConceptScore W2077400416C529278444 @default.
• W2077400416 hasConceptScore W2077400416C553089730 @default.
• W2077400416 hasConceptScore W2077400416C55493867 @default.
• W2077400416 hasConceptScore W2077400416C7821365 @default.
• W2077400416 hasConceptScore W2077400416C86803240 @default.

• next