Matches in SemOpenAlex for { <https://semopenalex.org/work/W2077414265> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2077414265 endingPage "146" @default.
- W2077414265 startingPage "137" @default.
- W2077414265 abstract "We describe the generation of mammalian cell lines carrying amber suppressor genes. Nonsense mutants in the herpes simplex virus thymidine kinase (HSV tk) gene, the Escherichia coli xanthine-guanine phosphoribosyl transferase (Eco-gpt) gene and the aminoglycoside 3′ phosphotransferase gene of the Tn5 transposon (NPT-II) were isolated and characterized. Each gene was engineered with the appropriate control signals to allow expression in both E. coli and mammalian cells. Expression in E. coli made possible the use of well developed bacterial and phage genetic manipulations to isolate and characterize the nonsense mutants. Once characterized, the nonsense mutants were transferred into mammalian cells by microinjection and used, in turn, to select for amber suppressor genes. Xenopus laevis amber suppressor genes, prepared by site-specific mutagenesis of a normal X. laevis tRNA gene, were microinjected into the above cell lines and selected for the expression of one or more of the amber mutant gene products. The resulting cell lines, containing functional amber suppressor genes, are stable and exhibit normal growth rates." @default.
- W2077414265 created "2016-06-24" @default.
- W2077414265 creator A5022945869 @default.
- W2077414265 creator A5054197881 @default.
- W2077414265 creator A5065080973 @default.
- W2077414265 creator A5075552021 @default.
- W2077414265 creator A5076354181 @default.
- W2077414265 date "1982-11-01" @default.
- W2077414265 modified "2023-10-18" @default.
- W2077414265 title "Establishment of mammalian cell lines containing multiple nonsense mutations and functional suppressor tRNA genes" @default.
- W2077414265 cites W1518139918 @default.
- W2077414265 cites W1555957867 @default.
- W2077414265 cites W1793444563 @default.
- W2077414265 cites W1966433657 @default.
- W2077414265 cites W1969152897 @default.
- W2077414265 cites W1980651107 @default.
- W2077414265 cites W1997654921 @default.
- W2077414265 cites W2000973771 @default.
- W2077414265 cites W2013388921 @default.
- W2077414265 cites W2016096268 @default.
- W2077414265 cites W2027932518 @default.
- W2077414265 cites W2031335201 @default.
- W2077414265 cites W2032258362 @default.
- W2077414265 cites W2048646033 @default.
- W2077414265 cites W2054631939 @default.
- W2077414265 cites W2056618475 @default.
- W2077414265 cites W2061555550 @default.
- W2077414265 cites W2073904950 @default.
- W2077414265 cites W2083589756 @default.
- W2077414265 cites W2105534447 @default.
- W2077414265 cites W2121349955 @default.
- W2077414265 cites W2335090692 @default.
- W2077414265 cites W4249051762 @default.
- W2077414265 doi "https://doi.org/10.1016/0092-8674(82)90413-5" @default.
- W2077414265 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6760983" @default.
- W2077414265 hasPublicationYear "1982" @default.
- W2077414265 type Work @default.
- W2077414265 sameAs 2077414265 @default.
- W2077414265 citedByCount "86" @default.
- W2077414265 countsByYear W20774142652012 @default.
- W2077414265 countsByYear W20774142652013 @default.
- W2077414265 countsByYear W20774142652015 @default.
- W2077414265 countsByYear W20774142652016 @default.
- W2077414265 countsByYear W20774142652018 @default.
- W2077414265 countsByYear W20774142652021 @default.
- W2077414265 crossrefType "journal-article" @default.
- W2077414265 hasAuthorship W2077414265A5022945869 @default.
- W2077414265 hasAuthorship W2077414265A5054197881 @default.
- W2077414265 hasAuthorship W2077414265A5065080973 @default.
- W2077414265 hasAuthorship W2077414265A5075552021 @default.
- W2077414265 hasAuthorship W2077414265A5076354181 @default.
- W2077414265 hasConcept C104317684 @default.
- W2077414265 hasConcept C143065580 @default.
- W2077414265 hasConcept C153911025 @default.
- W2077414265 hasConcept C26567756 @default.
- W2077414265 hasConcept C501734568 @default.
- W2077414265 hasConcept C54355233 @default.
- W2077414265 hasConcept C75563809 @default.
- W2077414265 hasConcept C86803240 @default.
- W2077414265 hasConcept C96777560 @default.
- W2077414265 hasConceptScore W2077414265C104317684 @default.
- W2077414265 hasConceptScore W2077414265C143065580 @default.
- W2077414265 hasConceptScore W2077414265C153911025 @default.
- W2077414265 hasConceptScore W2077414265C26567756 @default.
- W2077414265 hasConceptScore W2077414265C501734568 @default.
- W2077414265 hasConceptScore W2077414265C54355233 @default.
- W2077414265 hasConceptScore W2077414265C75563809 @default.
- W2077414265 hasConceptScore W2077414265C86803240 @default.
- W2077414265 hasConceptScore W2077414265C96777560 @default.
- W2077414265 hasIssue "1" @default.
- W2077414265 hasLocation W20774142651 @default.
- W2077414265 hasLocation W20774142652 @default.
- W2077414265 hasOpenAccess W2077414265 @default.
- W2077414265 hasPrimaryLocation W20774142651 @default.
- W2077414265 hasRelatedWork W101124211 @default.
- W2077414265 hasRelatedWork W1628900343 @default.
- W2077414265 hasRelatedWork W1897859244 @default.
- W2077414265 hasRelatedWork W2015472961 @default.
- W2077414265 hasRelatedWork W2044314002 @default.
- W2077414265 hasRelatedWork W2046946381 @default.
- W2077414265 hasRelatedWork W2117110650 @default.
- W2077414265 hasRelatedWork W2147466517 @default.
- W2077414265 hasRelatedWork W2372183968 @default.
- W2077414265 hasRelatedWork W2396837573 @default.
- W2077414265 hasVolume "31" @default.
- W2077414265 isParatext "false" @default.
- W2077414265 isRetracted "false" @default.
- W2077414265 magId "2077414265" @default.
- W2077414265 workType "article" @default.