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- W2077453675 abstract "Three methods are described for isolating HeLa cell nuclei using citric acid or Triton N-101 detergent. Two methods yielded nuclei relatively free of cytoplasmic contamination. Nuclei isolated in citric acid contained much less globulin and much more residual protein than nuclei isolated in detergent. Amino acid analysis indicated that the gloublins of HeLa cell nuclei contain relatively more basic amino acids than globulins from rat liver nuclei. The banding patterns of HeLa cell histones on acrylamide gels were similar to the pattern of calf thymus histones. Histones and residual protein from HeLa cell nuclei were similar in amino acid composition to the analogous fractions from rat liver nuclei." @default.
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- W2077453675 title "Nucleic acid and protein composition of hela cell nuclei isolated by three methods" @default.
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- W2077453675 doi "https://doi.org/10.1016/0014-4827(69)90453-4" @default.
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