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• W2077463432 endingPage "2915" @default.
• W2077463432 startingPage "2909" @default.
• W2077463432 abstract "The baculovirus expression system has been successfully used to overproduce a number of different protein products. In this report we describe the construction of a recombinant baculovirus containing the adenovirus E1A 13s cDNA sequence. Infection of Insect cells with this virus results in the production of phosphorylated E1A protein. The phosphorylatlon pattern appears to be similar to the complex pattern associated with E1A protein synthesis in mammalian cells. Purified baculovirus generated E1A protein activated transcription of specific pollll promoters both in microlnjected Xenopus laevis oocytes and in HeLa cell In vitro transcription extracts. The protein also stimulates in vitro transcription of the pollll transcribed VA1 gene." @default.
• W2077463432 created "2016-06-24" @default.
• W2077463432 creator A5076196099 @default.
• W2077463432 creator A5087776954 @default.
• W2077463432 date "1990-01-01" @default.
• W2077463432 modified "2023-09-25" @default.
• W2077463432 title "Activation<i>in vitro</i>of RNA polymerase II and III directed transcription by baculovirus produced E1A protein" @default.
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• W2077463432 doi "https://doi.org/10.1093/nar/18.10.2909" @default.
• W2077463432 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/330818" @default.
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• W2077463432 hasPublicationYear "1990" @default.
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