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• W2077481010 startingPage "97" @default.
• W2077481010 abstract "Polygalacturonase and α-amylase play vital role in fruit juice industry. In the present study, polygalacturonase was produced by Aspergillus awamori Nakazawa MTCC 6652 utilizing apple pomace and mosambi orange (Citrus sinensis var mosambi) peels as solid substrate whereas, α-amylase was produced from A. oryzae (IFO-30103) using wheat bran by solid state fermentation (SSF) process. These carbohydrases were decolourized and purified 8.6-fold, 34.8-fold and 3.5-fold, respectively by activated charcoal powder in a single step with 65.1%, 69.8% and 60% recoveries, respectively. Apple juice was clarified by these decolourized and partially purified enzymes. In presence of 1% polygalacturonase from mosambi peels (9.87 U/mL) and 0.4% α-amylase (899 U/mL), maximum clarity (%T(660 nm) = 97.0%) of juice was attained after 2 h of incubation at 50 °C in presence of 10 mM CaCl2. Total phenolic content of juice was reduced by 19.8% after clarification, yet with slightly higher %DPPH radical scavenging property." @default.
• W2077481010 created "2016-06-24" @default.
• W2077481010 creator A5074664699 @default.
• W2077481010 creator A5080376509 @default.
• W2077481010 date "2014-01-01" @default.
• W2077481010 modified "2023-10-12" @default.
• W2077481010 title "Application of decolourized and partially purified polygalacturonase and &#945;-amylase in apple juice clarification" @default.
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• W2077481010 doi "https://doi.org/10.1590/s1517-83822014000100014" @default.
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