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• W2077483937 abstract "The mitochondria of the respiratory yeast Kluyveromyces lactis are able to reoxidize cytosolic NADPH. Previously, we characterized an external alternative dehydrogenase, KlNde1p, having this activity. We now characterize the second external alternative dehydrogenase of K. lactis mitochondria, KlNde2p. We examined its role in cytosolic NADPH reoxidation by studying heterologous expression of KlNDE2 in Saccharomyces cerevisiae mutants and by constructing Deltaklnde1 and Deltaklnde2 mutants. KlNde2p uses NADH or NADPH as substrates, its activity in isolated mitochondria is not regulated by exogenously added calcium and it is not down-regulated when the cells grow in glucose versus lactate. KlNde2p shows lower affinity for NADPH than KlNde1p. Both enzymes show similar pH optimum." @default.
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• W2077483937 date "2006-11-01" @default.
• W2077483937 modified "2023-09-26" @default.
• W2077483937 title "Characterization of the second external alternative dehydrogenase from mitochondria of the respiratory yeast Kluyveromyces lactis" @default.
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• W2077483937 doi "https://doi.org/10.1016/j.bbabio.2006.09.002" @default.
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