FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2077515150> ?p ?o ?g. }

• W2077515150 endingPage "1354" @default.
• W2077515150 startingPage "1345" @default.
• W2077515150 abstract "For current tissue engineering or regenerative medicine strategies, chondrocyte (CC)- or mesenchymal stem cell (MSC)-seeded constructs are typically cultured in normoxic conditions (20% oxygen). However, within the knee joint capsule a lower oxygen tension exists.The objective of this study was to investigate how CCs and infrapatellar fad pad derived MSCs will respond to a low oxygen (5%) environment in 3D agarose culture. Our hypothesis was that culture in a low oxygen environment (5%) will enhance the functional properties of cartilaginous tissues engineered using both cell sources.Cell-encapsulated agarose hydrogel constructs (seeded with CCs or infrapatellar fat pad (IFP) derived MSCs) were prepared and cultured in a chemically defined serum-free medium in the presence (CCs and MSCs) or absence (CCs only) of transforming growth factor-beta3 (TGF-β3) in normoxic (20%) or low oxygen (5%) conditions for 42 days. Constructs were assessed at days 0, 21 and 42 in terms of mechanical properties, biochemical content and histologically.Low oxygen tension (5%) was observed to promote extracellular matrix (ECM) production by CCs cultured in the absence of TGF-β3, but was inhibitory in the presence of TGF-β3. In contrast, a low oxygen tension enhanced chondrogenesis of IFP constructs in the presence of TGF-β3, leading to superior mechanical functionality compared to CCs cultured in identical conditions.Extrapolating the results of this study to the in vivo setting, it would appear that joint fat pad derived MSCs may possess a superior potential to generate a functional repair tissue in low oxygen tensions. However, in the context of in vitro cartilage tissue engineering, CCs maintained in normoxic conditions in the presence of TGF-β3 generate the most mechanically functional tissue." @default.
• W2077515150 created "2016-06-24" @default.
• W2077515150 creator A5023142004 @default.
• W2077515150 creator A5045482826 @default.
• W2077515150 creator A5081947117 @default.
• W2077515150 date "2010-10-01" @default.
• W2077515150 modified "2023-10-13" @default.
• W2077515150 title "Oxygen tension differentially regulates the functional properties of cartilaginous tissues engineered from infrapatellar fat pad derived MSCs and articular chondrocytes" @default.
• W2077515150 cites W104976773 @default.
• W2077515150 cites W1522229466 @default.
• W2077515150 cites W1544751778 @default.
• W2077515150 cites W1561858686 @default.
• W2077515150 cites W1965075111 @default.
• W2077515150 cites W1965380417 @default.
• W2077515150 cites W1965672870 @default.
• W2077515150 cites W1967366984 @default.
• W2077515150 cites W1968217647 @default.
• W2077515150 cites W1968222609 @default.
• W2077515150 cites W1968582434 @default.
• W2077515150 cites W1970692048 @default.
• W2077515150 cites W1972349420 @default.
• W2077515150 cites W1974128523 @default.
• W2077515150 cites W1978772690 @default.
• W2077515150 cites W1980114823 @default.
• W2077515150 cites W1985286917 @default.
• W2077515150 cites W1991415492 @default.
• W2077515150 cites W1992598304 @default.
• W2077515150 cites W1992739919 @default.
• W2077515150 cites W1995122819 @default.
• W2077515150 cites W1996087228 @default.
• W2077515150 cites W1997660384 @default.
• W2077515150 cites W2001498622 @default.
• W2077515150 cites W2002100798 @default.
• W2077515150 cites W2002963723 @default.
• W2077515150 cites W2004505453 @default.
• W2077515150 cites W2007469598 @default.
• W2077515150 cites W2008599030 @default.
• W2077515150 cites W2010304813 @default.
• W2077515150 cites W2013148027 @default.
• W2077515150 cites W2013682631 @default.
• W2077515150 cites W2020342708 @default.
• W2077515150 cites W2023026891 @default.
• W2077515150 cites W2023436570 @default.
• W2077515150 cites W2023550825 @default.
• W2077515150 cites W2026220322 @default.
• W2077515150 cites W2026498351 @default.
• W2077515150 cites W2035105700 @default.
• W2077515150 cites W2035528429 @default.
• W2077515150 cites W2038611660 @default.
• W2077515150 cites W2040035248 @default.
• W2077515150 cites W2043814008 @default.
• W2077515150 cites W2045413421 @default.
• W2077515150 cites W2046927280 @default.
• W2077515150 cites W2048037026 @default.
• W2077515150 cites W2050273631 @default.
• W2077515150 cites W2050440015 @default.
• W2077515150 cites W2051733665 @default.
• W2077515150 cites W2051845426 @default.
• W2077515150 cites W2052620919 @default.
• W2077515150 cites W2056361979 @default.
• W2077515150 cites W2056951922 @default.
• W2077515150 cites W2062279390 @default.
• W2077515150 cites W2073780395 @default.
• W2077515150 cites W2074098728 @default.
• W2077515150 cites W2074121232 @default.
• W2077515150 cites W2076542802 @default.
• W2077515150 cites W2079295507 @default.
• W2077515150 cites W2079416369 @default.
• W2077515150 cites W2086236912 @default.
• W2077515150 cites W2086862250 @default.
• W2077515150 cites W2091464294 @default.
• W2077515150 cites W2092771822 @default.
• W2077515150 cites W2093453395 @default.
• W2077515150 cites W2101471690 @default.
• W2077515150 cites W2105389636 @default.
• W2077515150 cites W2115465747 @default.
• W2077515150 cites W2118566351 @default.
• W2077515150 cites W2118700761 @default.
• W2077515150 cites W2125531516 @default.
• W2077515150 cites W2131456542 @default.
• W2077515150 cites W2136670166 @default.
• W2077515150 cites W2137087836 @default.
• W2077515150 cites W2138007542 @default.
• W2077515150 cites W2151378125 @default.
• W2077515150 cites W2152606567 @default.
• W2077515150 cites W2158640783 @default.
• W2077515150 cites W2159999899 @default.
• W2077515150 cites W2172031022 @default.
• W2077515150 cites W2173528967 @default.
• W2077515150 cites W2184277544 @default.
• W2077515150 cites W2288473469 @default.
• W2077515150 doi "https://doi.org/10.1016/j.joca.2010.07.004" @default.
• W2077515150 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20650328" @default.
• W2077515150 hasPublicationYear "2010" @default.
• W2077515150 type Work @default.
• W2077515150 sameAs 2077515150 @default.
• W2077515150 citedByCount "93" @default.
• W2077515150 countsByYear W20775151502012 @default.

• next