FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2077560184> ?p ?o ?g. }

• W2077560184 endingPage "66" @default.
• W2077560184 startingPage "53" @default.
• W2077560184 abstract "The response of protein accumulation site on yield, biological activity and in planta stability of therapeutic recombinant human α1-proteinase inhibitor (α1-PI) was analyzed via targeting to different subcellular locations, like endoplasmic reticulum (ER), apoplast, vacuole and cytosol in leaves of transgenic tomato plants. In situ localization of the recombinant α1-PI protein in transgenic plant cells was monitored by immunohistochemical staining. Maximum accumulation of recombinant α1-PI in T0 and T1 transgenic tomato plants was achieved from 1.5 to 3.2% of total soluble protein (TSP) by retention in ER lumen, followed by vacuole and apoplast, whereas cytosolic targeting resulted into degradation of the protein. The plant-derived recombinant α1-PI showed biological activity for elastase inhibition, as monitored by residual porcine pancreatic elastase (PPE) activity assay and band-shift assay. Recombinant α1-PI was purified from transgenic tomato plants with high yield, homogeneity and biological activity. Purified protein appeared as a single band of ∼48–50 kDa on SDS-PAGE with pI value ranging between 5.1 and 5.3. Results of mass spectrometry and optical spectroscopy of purified recombinant α1-PI revealed the structural integrity of the recombinant protein comparable to native serum α1-PI. Enzymatic deglycosylation and lectin-binding assays with the purified recombinant α1-PI showed compartment-specific N-glycosylation of the protein targeted to ER, apoplast and vacuole. Conformational studies based on urea-induced denaturation and circular dichroism (CD) spectroscopy revealed relatively lower stability of the recombinant α1-PI protein, compared to its serum counterpart. Pharmacokinetic evaluation of plant derived recombinant and human plasma-purified α1-PI in rat, by intravenous route, revealed significantly faster plasma clearance and lower area under curve (AUC) of recombinant protein. Our data suggested significance of protein sorting sequences and feasibility to use transgenic plants for the production of stable, glycosylated and biologically active recombinant α1-PI for further therapeutic applications." @default.
• W2077560184 created "2016-06-24" @default.
• W2077560184 creator A5014169797 @default.
• W2077560184 creator A5015084810 @default.
• W2077560184 creator A5029955399 @default.
• W2077560184 creator A5060377718 @default.
• W2077560184 creator A5062491157 @default.
• W2077560184 date "2012-11-01" @default.
• W2077560184 modified "2023-09-27" @default.
• W2077560184 title "Differential subcellular targeting of recombinant human α1-proteinase inhibitor influences yield, biological activity and in planta stability of the protein in transgenic tomato plants" @default.
• W2077560184 cites W1519781060 @default.
• W2077560184 cites W1601649545 @default.
• W2077560184 cites W1678784904 @default.
• W2077560184 cites W1814267858 @default.
• W2077560184 cites W1878547122 @default.
• W2077560184 cites W1893730694 @default.
• W2077560184 cites W1985492583 @default.
• W2077560184 cites W1991366673 @default.
• W2077560184 cites W1993575547 @default.
• W2077560184 cites W1997191182 @default.
• W2077560184 cites W1997607253 @default.
• W2077560184 cites W2002062036 @default.
• W2077560184 cites W2004538569 @default.
• W2077560184 cites W2007069042 @default.
• W2077560184 cites W2011326234 @default.
• W2077560184 cites W2017756393 @default.
• W2077560184 cites W2018604605 @default.
• W2077560184 cites W2026760618 @default.
• W2077560184 cites W2027105364 @default.
• W2077560184 cites W2028629349 @default.
• W2077560184 cites W2034768596 @default.
• W2077560184 cites W2035851443 @default.
• W2077560184 cites W2042150148 @default.
• W2077560184 cites W2049537986 @default.
• W2077560184 cites W2051313463 @default.
• W2077560184 cites W2059849821 @default.
• W2077560184 cites W2060037459 @default.
• W2077560184 cites W2066250918 @default.
• W2077560184 cites W2069275136 @default.
• W2077560184 cites W2069277611 @default.
• W2077560184 cites W2076875064 @default.
• W2077560184 cites W2091935675 @default.
• W2077560184 cites W2093605720 @default.
• W2077560184 cites W2103456166 @default.
• W2077560184 cites W2103986567 @default.
• W2077560184 cites W2116076986 @default.
• W2077560184 cites W2120095650 @default.
• W2077560184 cites W2126104240 @default.
• W2077560184 cites W2129168757 @default.
• W2077560184 cites W2132177439 @default.
• W2077560184 cites W2135293202 @default.
• W2077560184 cites W2138484187 @default.
• W2077560184 cites W2140035322 @default.
• W2077560184 cites W2140210459 @default.
• W2077560184 cites W2142297287 @default.
• W2077560184 cites W2159423922 @default.
• W2077560184 cites W2165175234 @default.
• W2077560184 cites W2165832011 @default.
• W2077560184 doi "https://doi.org/10.1016/j.plantsci.2012.07.004" @default.
• W2077560184 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23017899" @default.
• W2077560184 hasPublicationYear "2012" @default.
• W2077560184 type Work @default.
• W2077560184 sameAs 2077560184 @default.
• W2077560184 citedByCount "23" @default.
• W2077560184 countsByYear W20775601842013 @default.
• W2077560184 countsByYear W20775601842014 @default.
• W2077560184 countsByYear W20775601842015 @default.
• W2077560184 countsByYear W20775601842016 @default.
• W2077560184 countsByYear W20775601842017 @default.
• W2077560184 countsByYear W20775601842018 @default.
• W2077560184 countsByYear W20775601842019 @default.
• W2077560184 countsByYear W20775601842020 @default.
• W2077560184 countsByYear W20775601842021 @default.
• W2077560184 countsByYear W20775601842022 @default.
• W2077560184 crossrefType "journal-article" @default.
• W2077560184 hasAuthorship W2077560184A5014169797 @default.
• W2077560184 hasAuthorship W2077560184A5015084810 @default.
• W2077560184 hasAuthorship W2077560184A5029955399 @default.
• W2077560184 hasAuthorship W2077560184A5060377718 @default.
• W2077560184 hasAuthorship W2077560184A5062491157 @default.
• W2077560184 hasConcept C102568950 @default.
• W2077560184 hasConcept C104317684 @default.
• W2077560184 hasConcept C123894998 @default.
• W2077560184 hasConcept C125235067 @default.
• W2077560184 hasConcept C136345775 @default.
• W2077560184 hasConcept C153911025 @default.
• W2077560184 hasConcept C190062978 @default.
• W2077560184 hasConcept C40767141 @default.
• W2077560184 hasConcept C55493867 @default.
• W2077560184 hasConcept C86803240 @default.
• W2077560184 hasConceptScore W2077560184C102568950 @default.
• W2077560184 hasConceptScore W2077560184C104317684 @default.
• W2077560184 hasConceptScore W2077560184C123894998 @default.
• W2077560184 hasConceptScore W2077560184C125235067 @default.
• W2077560184 hasConceptScore W2077560184C136345775 @default.
• W2077560184 hasConceptScore W2077560184C153911025 @default.
• W2077560184 hasConceptScore W2077560184C190062978 @default.
• W2077560184 hasConceptScore W2077560184C40767141 @default.

• next