FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2077576278> ?p ?o ?g. }

• W2077576278 endingPage "42776" @default.
• W2077576278 startingPage "42770" @default.
• W2077576278 abstract "Enzymological paradigms have shifted recently to acknowledge the biological importance of catalytic promiscuity. However, catalytic promiscuity is a poorly understood property, and no thermodynamic treatment has described the conformational landscape of promiscuous versus substrate-specific enzymes. Here, two structurally similar glutathione transferase (GST, glutathione S-transferase) isoforms with high specificity or high promiscuity are compared. Differential scanning calorimetry (DSC) indicates a reversible low temperature transition for the promiscuous GSTA1-1 that is not observed with substrate-specific GSTA4-4. This transition is assigned to rearrangement of the C terminus at the active site of GSTA1-1 based on the effects of ligands and mutations. Near-UV and far-UV circular dichroism indicate that this transition is due to repacking of tertiary contacts with the remainder of the subunit, rather than unfolding of the C terminus per se. Analysis of the DSC data using a modified Landau theory indicates that the local conformational landscape of the active site of GSTA1-1 is smooth, with barrierless transitions between states. The partition function of the C-terminal states is a broad unimodal distribution at all temperatures within this DSC transition. In contrast, the remainder of the GSTA1-1 subunit and the GSTA4-4 protein exhibit folded and unfolded macrostates with a significant energy barrier separating them. Their partition function includes a sharp unimodal distribution of states only at temperatures that yield either folded or unfolded macrostates. At intermediate temperatures the partition function includes a bimodal distribution. The barrierless rearrangement of the GSTA1-1 active site within a local smooth energy landscape suggests a thermodynamic basis for catalytic promiscuity." @default.
• W2077576278 created "2016-06-24" @default.
• W2077576278 creator A5014425767 @default.
• W2077576278 creator A5070378701 @default.
• W2077576278 creator A5080008157 @default.
• W2077576278 creator A5082840398 @default.
• W2077576278 date "2011-12-01" @default.
• W2077576278 modified "2023-10-14" @default.
• W2077576278 title "Ensemble Perspective for Catalytic Promiscuity" @default.
• W2077576278 cites W1572376186 @default.
• W2077576278 cites W1581414347 @default.
• W2077576278 cites W1788236687 @default.
• W2077576278 cites W1964126053 @default.
• W2077576278 cites W1969766098 @default.
• W2077576278 cites W1970195807 @default.
• W2077576278 cites W1971007618 @default.
• W2077576278 cites W1972662391 @default.
• W2077576278 cites W1980527476 @default.
• W2077576278 cites W1982344941 @default.
• W2077576278 cites W1985349154 @default.
• W2077576278 cites W1990357483 @default.
• W2077576278 cites W1992625839 @default.
• W2077576278 cites W1998238795 @default.
• W2077576278 cites W2004909324 @default.
• W2077576278 cites W2006064320 @default.
• W2077576278 cites W2007384131 @default.
• W2077576278 cites W2010224035 @default.
• W2077576278 cites W2015802360 @default.
• W2077576278 cites W2025785557 @default.
• W2077576278 cites W2028767577 @default.
• W2077576278 cites W2030081928 @default.
• W2077576278 cites W2036985028 @default.
• W2077576278 cites W2039369222 @default.
• W2077576278 cites W2047696147 @default.
• W2077576278 cites W2049888624 @default.
• W2077576278 cites W2051481147 @default.
• W2077576278 cites W2054118966 @default.
• W2077576278 cites W2055982953 @default.
• W2077576278 cites W2056765995 @default.
• W2077576278 cites W2058627227 @default.
• W2077576278 cites W2061634201 @default.
• W2077576278 cites W2062501429 @default.
• W2077576278 cites W2079794273 @default.
• W2077576278 cites W2088563323 @default.
• W2077576278 cites W2092759716 @default.
• W2077576278 cites W2102549899 @default.
• W2077576278 cites W2106531843 @default.
• W2077576278 cites W2128312954 @default.
• W2077576278 cites W2130983257 @default.
• W2077576278 cites W2132524280 @default.
• W2077576278 cites W2150790322 @default.
• W2077576278 cites W2152257846 @default.
• W2077576278 cites W2160294964 @default.
• W2077576278 cites W2164732543 @default.
• W2077576278 cites W2171551395 @default.
• W2077576278 cites W4234732727 @default.
• W2077576278 cites W4238581030 @default.
• W2077576278 cites W4249435533 @default.
• W2077576278 doi "https://doi.org/10.1074/jbc.m111.304386" @default.
• W2077576278 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3234982" @default.
• W2077576278 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22002059" @default.
• W2077576278 hasPublicationYear "2011" @default.
• W2077576278 type Work @default.
• W2077576278 sameAs 2077576278 @default.
• W2077576278 citedByCount "29" @default.
• W2077576278 countsByYear W20775762782012 @default.
• W2077576278 countsByYear W20775762782013 @default.
• W2077576278 countsByYear W20775762782014 @default.
• W2077576278 countsByYear W20775762782015 @default.
• W2077576278 countsByYear W20775762782017 @default.
• W2077576278 countsByYear W20775762782018 @default.
• W2077576278 countsByYear W20775762782019 @default.
• W2077576278 countsByYear W20775762782020 @default.
• W2077576278 countsByYear W20775762782021 @default.
• W2077576278 countsByYear W20775762782022 @default.
• W2077576278 countsByYear W20775762782023 @default.
• W2077576278 crossrefType "journal-article" @default.
• W2077576278 hasAuthorship W2077576278A5014425767 @default.
• W2077576278 hasAuthorship W2077576278A5070378701 @default.
• W2077576278 hasAuthorship W2077576278A5080008157 @default.
• W2077576278 hasAuthorship W2077576278A5082840398 @default.
• W2077576278 hasBestOaLocation W20775762781 @default.
• W2077576278 hasConcept C104292427 @default.
• W2077576278 hasConcept C104317684 @default.
• W2077576278 hasConcept C119621388 @default.
• W2077576278 hasConcept C121332964 @default.
• W2077576278 hasConcept C12554922 @default.
• W2077576278 hasConcept C133571119 @default.
• W2077576278 hasConcept C181199279 @default.
• W2077576278 hasConcept C185592680 @default.
• W2077576278 hasConcept C2776376580 @default.
• W2077576278 hasConcept C39519442 @default.
• W2077576278 hasConcept C41183919 @default.
• W2077576278 hasConcept C55493867 @default.
• W2077576278 hasConcept C71240020 @default.
• W2077576278 hasConcept C8010536 @default.
• W2077576278 hasConcept C86803240 @default.
• W2077576278 hasConcept C97355855 @default.

• next