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- W2077593313 abstract "Abstract When breaks are introduced into double-stranded covalently closed circular sex-factor DNA molecules, the complementary strands can be separated in a CsCl-poly (U, G) equilibrium density gradient. Using this technique, double-stranded circular sex-factor DNA isolated from donor and recipient cells after mating was analyzed to determine the fate of the complementary strands. Only one strand of the sex-factor DNA is transferred from donor to recipient cells, this being the denser strand in CsCl-poly (U, G). A complement to this strand is synthesized in the recipient and a covalently closed sex-factor DNA molecule is formed. The sex-factor strand not transferred to the recipient remains in the donor where it acquires a complement during mating and forms a covalently closed double-stranded circle. These results show that DNA synthesis associated with mating occurs in both the donor and recipient cells. The asymmetric segregation of the complementary sex-factor DNA strands could be generated by a mechanism such as the rolling circle model of DNA synthesis proposed by Gilbert & Dressler (1968)." @default.
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- W2077593313 date "1970-11-01" @default.
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- W2077593313 title "Asymmetric segregation of the complementary sex-factor DNA strands during conjugation in Escherichia coli" @default.
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- W2077593313 doi "https://doi.org/10.1016/0022-2836(70)90066-5" @default.
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