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- W2077641310 abstract "Preparations of normal rabbit IgG antibody (NgG) and purified rabbit IgG antibody of anti-2,4-dinitrophenyl (Anti-Dnp) specificity were covantly labeled with the flourescent probe 2-dimethylaminonaphthalene-5-sulfonyl chloride (2,5-Dns-Cl). 2,5-Dns-immunoglobulin G preparations were shown to have average flourescence lifetimes of 29–32 ns by cross-correlated phase and modulation flourescence lifetime measurements. Excitation maxima were observed at 275 and 368 nm, while the emission maximum occured at 465 nm. With 390 nm excitation, an intrinsic flourescence polarization of 0.43 was observed. Molar extinction coefficients of 36 000 M−1·cm−1 (at 258 nm) and 2090 M−1·−1 (at 352 nm) were determined by synthesis of 2,5-DNS-L-[4,5-3H]leucine, a tritiated sulfonomide of 2,5-DNS. Low level labelling (1 mol 2,5-Dns/mol of antibody) of rabbit Anti-Dnp IgG antibody with 2,5-DNS-Cl did not significantly alter the antibody association constant with Dnp. Fluorescence polarization measurements were accurately measured (0.3% error) at antibody concentrations greater than 10−7 M, using photon counting instrumentation. Rotational relaxation times of 128 and 131 ns were determined for 2,5-DNS-NgG and 2,5-Dns-Anti-Dnp, respectively." @default.
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- W2077641310 date "1981-07-01" @default.
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- W2077641310 title "Characterization of flourescent 2-dimethylaminonaphthalene-5-sulfonyl-immunoglobulin G conjugates for application in fluorescence polarization studies" @default.
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- W2077641310 doi "https://doi.org/10.1016/0165-022x(81)90029-4" @default.
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