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- W2077677191 abstract "Long-term cultures of human lymphoid lines, even after subcloning, were found to contain a mixture of surface immunoglobulin (SIg) positive and negative cells using three different techniques. Cell culture (TM) was mixed with anti-Ig antisera plus complement to lyse SIg positive cells. The remaining SIg negative viable cells were separated on a bovine serum albumin gradient, cultured, and sampled at different time points. Within 24–48 hr the same percentage of cells were SIg(+) as prior to treatment with antisera plus complement. Culturing the same cells with frequent additions of fresh or “spent” culture media failed to alter the ratio of Ig positive/ negative cells. Mechanisms for in vitro regulation of SIg are discussed." @default.
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- W2077677191 title "Phenotypic heterogeneity for cells bearing surface immunoglobulin in human lymphoid lines" @default.
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- W2077677191 doi "https://doi.org/10.1016/0008-8749(76)90211-2" @default.
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